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董海滨,任 伟,韩留鹏,昝香存,赵明忠,王松峰,高 崇,张 煜.河南省小麦品种(系)粒重相关基因等位变异的鉴定与分析[J].麦类作物学报,2024,(8):996
河南省小麦品种(系)粒重相关基因等位变异的鉴定与分析
Identification and Analysis of Allelic Variation Types of Grain Weight Related Genes in Henan Wheat Varieties (Lines)
  
DOI:
中文关键词:  河南  小麦  粒重基因  等位变异  千粒重
英文关键词:Henan  Wheat  Grain weight genes  Allelic variation  Thousand-grain weight
基金项目:河南省产业研发联合基金重大项目(225101610048)
作者单位
董海滨,任 伟,韩留鹏,昝香存,赵明忠,王松峰,高 崇,张 煜 (河南省作物分子育种研究院/农业部黄淮中部小麦生物学与遗传育种重点实验室/河南省麦类种质资源创新与改良重点实验室河南郑州 450002) 
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中文摘要:
      小麦粒重是影响小麦产量的重要因素之一,培育高粒重品种是小麦育种的重要目标之一。本研究以395份河南省小麦品种(系)为材料,结合2021-2023连续两年千粒重测定结果,利用分子标记技术对其粒重相关基因(TaCwi-A1TaSus2-2BTaGw8-B1TaGS-D1)的等位变异进行了鉴定,分析4种粒重基因不同等位变异及其不同组合类型对千粒重的影响。结果表明,TaCwi-A1TaSus2-2BTaGw8-B1TaGS-D1位点在供试材料中均存在2种等位变异,共具有15种不同粒重等位基因组合类型。综合分析不同粒重等位基因及其组合类型与千粒重关系,等位基因TaSus2-2Ba对粒重的正向作用效应显著,而等位基因TaCwi-A1aTaGS-D1a分别对粒重的正向效应不显著。TaCwi-A1b+TaSus2-2Ba+TaGS-D1b+TaGw8-BlaTaCwi-A1a+TaSus2-2Ba+TaGS-D1a+TaGw8-B1aTaCwi-A1b+TaSus2-2Ba+TaGS-D1a+TaGw8-B1a这3种基因组合类型为小麦高粒重优势基因型。
英文摘要:
      Wheat grain weight is one of the important factors of wheat yield, and cultivating varieties with high grain weight is an important goal of high-yield wheat breeding. In this study, 395 wheat varieties (lines) from Henan Province were used to identify the allelic variations of grain weight related genes TaCwi-A1, TaSus2-2B, TaGw8-B1, and TaGS-D1. At the same time, the thousand-grain weight of these materials were measured for two consecutive years from 2021 to 2023, in order to further analyze the effects of different allele variations and combinations of the above-mentioned four grain weight genes on thousand-grain weight. The results showed that the loci of TaCwi-A1, TaSus2-2B, TaGw8-B1, and TaGS-D1 all had two allelic variations, and 15 different combinations of the grain weight alleles in 395 wheat varieties (lines) were identified. It was found that the TaSus2-2Ba had a significant positive effect on grain weight, while the TaCwi-A1a and TaGS-D1a alleles had no significant positive effect on grain weight. Varieties (lines) with the three genotypes of different allele combinations:TaCwi-A1b+TaSus2-2Ba+TaGS-D1b+TaGw8-B1a, TaCwi-A1a+TaSus2-2Ba+TaGS-D1a+TaGw8-B1a, and TaCwi-A1b+TaSus2-2Ba+TaGS-D1a+TaGw8-B1a, had a relatively high average thousand-grain weight, which are the prior genotypes.
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