The precision of crop variety regional trials is the foundation of candidate variety evaluation and accurate selection. This study analyzed the experiment precision (EP) and variety comparison precision (VCP) of single and multiple-location wheat regional trials in the winter wheat region of the Yangtze River Valley (YRV) from 2011 to 2021, and compared EP andVCP of multiple traits including grain yield, spike number, kernel number per spike, 1 000-kernel weight, growth duration, plant height, basic seedling number, and maximum stem and tillers. Results showed that: EP of the single wheat trials in the winter wheat region of YRV performed well enough. The proportion of single trials with the coefficient of error variation (CEV) less than 5% and 10% was above 60% and 95%, respectively. However, the proportion of single trials with the relative least significant difference at 0.05 significant level (RLSD0.05) below 3% is less than 10%, and those with RLSD0.05 below 10% in the upper, and middle and lower reaches of YRV accounted only for about 60% and 90%, respectively.EP and VCP of one-year multi-location wheat trials were significantly better than those of single trials. The average CEV of multi-location trials in one year in the upper, and middle and lower reaches of YRV was below 8% and 5%, respectively. Under the site fixed ANOVA model, the average RLSD0.05 in the upper reaches and the middle-lower reaches of YRV were 2.19% and 1.49%, respectively, which met the requirements of the national wheat registration standards in terms of variety comparison accuracy. Nevertheless, even the multi-location wheat trials in YRV were not sensitive enough to identify 3% yield difference among varieties under site random ANOVA mode.The estimated RLSD0.05 values of growth duration, plant height, basic seedling number, 1 000-kernel weight, and spike number were found better than that of grain yield, while RLSD0.05 of maximum stem and tillers, and kernel number per spike was relatively lower. |