| SQUAMOSA promoter binding protein like(SPL) gene family is a family of transcription factors widely existing in plants, and plays important roles in regulating plant growth and development and responses to stresses. In order to further analyze the mechanism of SPL gene family responding to abiotic stresses, bioinformatics methods were used to identify SPL family members in the wheat genome database, and the expression of the identified genes were analyzed under abiotic stress conditions such as nitrogen deficiency, phosphorus deficiency, high salt, low temperature, drought, and high temperature. The results showed that 56 SPL family genes were identified in wheat genome, and 27 of which were target genes of miR156. Phylogenetic analysis showed that 56 wheat SPL genes were clustered into 7 subfamilies. Based on transcriptome data, the expression patterns of 36 wheat SPL genes related to abiotic stress response were analyzed, and 12, 16, 22, 6, 13, 14 and 21 genes were found to respond to nitrogen deficiency, phosphorus deficiency, high salt, low temperature, drought, high temperature, and combination stress of high temperature and drought, respectively. TraesCS3D02G425800 responded to seven abiotic stresses simultaneously. The results of qRT-PCR were consistent with the transcriptome data. |
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