| 韦秀兰，苏 宁，刘 涛，聂小军，童 维.小麦SnRK2家族基因鉴定及进化分析[J].麦类作物学报,2023,(10):1326 |
| 小麦SnRK2家族基因鉴定及进化分析 |
| Identification of SnRK2 Gene Family and Evolution Analysis in Wheat |
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| DOI： |
| 中文关键词: 小麦 SnRK2基因 非生物胁迫 基因表达 |
| 英文关键词:Wheat SnRK2 gene Abiotic stress Gene expression |
| 基金项目:杨凌科技攻关项目（2016NY-17） |
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| 摘要点击次数: 80 |
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| 中文摘要: |
| SnRK2是一种植物特异性的丝氨酸/苏氨酸蛋白激酶，在植物生长发育和胁迫耐受信号传递过程中发挥着至关重要的作用。为探索SnRK2在小麦抗逆中的作用，基于最新的小麦基因组信息，采用blastp和hmmer两种方法在普通小麦中共鉴定到30个SnRK2基因家族成员。系统发育分析表明，SnRK2基因在普通小麦及其祖先物种的进化过程中高度保守。利用RNA seq数据分析小麦SnRK2基因在不同组织以及不同胁迫条件下的表达模式，结果表明，小麦SnRK2基因家族成员在不同组织以及不同胁迫条件下表达量均存在差异，且具有显著的组织特异性。通过qRT PCR进一步验证6个小麦SnRK2基因的表达模式，发现不同基因之间的表达量存在明显差异，推测小麦SnRK2基因家族的部分成员出现功能分化。随后，利用已发表的六倍体小麦重测序数据，分析不同小麦群体中SnRK2基因的核苷酸多样性(Pi)和分化指数(Fst)，并解析单倍型，推测Ta 5B SnRK2.28基因的AA单倍型为优异等位变异。通过同源建模预测小麦SnRK2蛋白的三维结构，发现小麦SnRK2蛋白结构在进化上高度保守。 |
| 英文摘要: |
| SnRK2 is a plant specific Ser/Thr protein kinase, which plays a crucial role in plant growth, development, and stress tolerance signaling pathways. To explore its role in wheat stress resistance, based on the latest wheat genome information, 30 SnRK2 genes were identified using blastp and hmmer methods in wheat.Phylogenetic tree analysis showed that wheat SnRK2 genes were highly conserved in the evolution of common wheat and its ancestor species.RNA-seq data were utilized to analyze the expression of wheat SnRK2 gene in different tissues and different stress conditions. The results indicated that the wheat SnRK2 genes were differentially expressed in different tissues and different stress conditions, with obvious tissue specificity. The expression of six wheat SnRK2 genes was further verified by qRT PCR, and significant differences were found among the homologous genes.It is speculated that some members of the SnRK2 gene family in wheat are functionally differentiated. Then, using published resequencing data of hexaploid wheat, we calculated the Pi and Fst of the SnRK2 genes regions in each population and statistically analyzed the haplotype. We speculated that AA haplotype of Ta 5B SnRK2.28 gene is a superior allelic variation. 3D structures of the wheat SnRK2 proteins were predicted by homology modeling, which indicated that the structure of SnRK2 proteins was highly conservative. |
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