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作者	单位
余燕，周茂润，董嘉，丁茂文，岳文丽，周可金，孙成亮，林咸永	(1.安徽农业大学农学院，安徽合肥 230036；2.浙江大学环境与资源学院，浙江杭州 310058）

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中文摘要:

为探明铝胁迫下多胺种类和含量变化与小麦耐铝性的关系，以小麦耐铝基因型西矮麦1号和铝敏感基因型扬麦5号为试验材料，分析了对照和铝胁迫（30 μmol·L^-1 AlCl₃）条件下，两个小麦基因型幼苗根尖多胺种类和含量、根系伸长、氧化损伤和活性氧产生等指标的差异。结果表明，随铝胁迫时间的延长，小麦幼苗根系伸长量逐渐降低，但西矮麦1号显著高于扬麦5号。铝胁迫24 h后，2个小麦基因型根尖铝含量、膜脂过氧化程度、Evans blue吸收量、活性氧含量均显著升高，且西矮麦1号各指标均显著低于扬麦5号。铝胁迫下2个小麦基因型根尖腐胺总量随铝胁迫时间的延长均不同程度增加，西矮麦1号根尖腐胺总量增加时间明显早于扬麦1号，且前者增加幅度明显高于后者。相反，根尖亚精胺总量随铝胁迫时间的延长显著降低，西矮麦1号的降幅明显小于扬麦5号。添加腐胺合成抑制剂（D-Arg）显著加剧了铝诱导的氧化损伤，且对西矮麦1号的加剧作用较明显。这说明铝诱导的小麦多胺水平变化与耐铝性密切相关，腐胺总量的增加可能是耐铝的重要机制，而亚精胺总量的降低可能与小麦铝敏感性有关。

英文摘要:

To investigate the relationship between polyamines change profiles and aluminum(Al) tolerance in wheat seedlings,an Al tolerant wheat genotype Xiaimai 1 and an Al sensitive genotype Yangmai 5 were used as materials. The variations of polyamine levels,root elongation,oxidative damage,and reactive oxygen species(ROS) of the two wheat genotypes under 30 μmol·L^-1 Al stress were studied. The results showed that the root elongation of wheat seedlings decreased gradually with the prolongation of aluminum stress time,but the root elongation of Xiaimai 1 was significantly higher than that of Yangmai 5. After aluminum stress for 24 h,aluminum content,membrane lipid peroxidation,Evans blue uptake,and ROS content in root tips of the two wheat genotypes were significantly increased,and all indicators of Xiaimai 1 were significantly lower than those of Yangmai 5. Under aluminum stress,the total amount of putrescine in root tips of the two wheat genotypes increased in varying degrees with the extension of aluminum stress time. The total amount of putrescine in root tips of Xiaimai 1 increased earlier than that of Yangmai 1,and the increase rate of the former was significantly higher than that of the latter. On the contrary,the total amount of spermidine in root tips decreased significantly with the prolongation of aluminum stress time,and the decrease rate of Xiaimai 1 was significantly less than that of Yangmai 5. The application of putrescine synthesis inhibitor (D-Arg) significantly aggravated the Al induced oxidative damage in the two wheat genotypes,and the effect on Xiaimai 1 was more obvious. These results suggest that the change of polyamines level induced by aluminum was closely related to aluminum tolerance of wheat. The increase of putrescine amount might be an important mechanism of aluminum tolerance,while the decrease of spermidine amount might be related to aluminum sensitivity of wheat.

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