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作者	单位
董娜，王玉泉，陈向东，胡铁柱，吴晓军，张亚娟，茹振钢	（河南科技学院小麦中心/河南省杂交小麦重点实验室/现代生物育种河南省协同创新中心，河南新乡453003）

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中文摘要:

为明确39份外引小麦种质的抗叶锈病基因组成，利用与抗病基因紧密连锁或共分离的分子标记对抗叶锈病基因进行检测，并结合田间接种鉴定对种质材料的叶锈病抗性进行评价。结果表明，抗叶锈病基因Lr1、Lr10、Lr20、Lr24、Lr26、Lr34、Lr37和Lr38在39份种质中均有分布，频率分别为30.77%、17.95%、2.56%、5.13%、12.82%、30.77%、23.08%和5.13%，其中携带Lr24和Lr38的种质抗性良好。Lr34和Lr37单独存在时，种质对叶锈病的抗性分别表现为中感和高感，两者聚合时能够提高载体种质的抗性水平，表现为中抗。此外，4份种质材料（KPL-1、OK.95571、莫斯科39和非黑土地24）对叶锈病的抗性表现突出，均达到0；级，是抗叶锈病育种的重要抗源，其中KPL-1和OK.95571分别携带3个和5个抗叶锈病基因；莫斯科39和非黑土地24仅携带Lr1，推测这两份材料含有其他未检测的抗叶锈病基因。综上，较多的抗叶锈病基因聚合有利于提高种质的叶锈病抗性。

英文摘要:

In order to clarify the composition of leaf rust resistance genes in 39 introduced wheat germplasms, the disease-resistant genes were detected with tightly-linked or co-segregated markers, and the disease resistant level of these germplasms were evaluated by artificial inoculation in the field. The results showed that the leaf rust resistance genes Lr1, Lr10, Lr20, Lr24, Lr26, Lr34, Lr37, and Lr38 〖JP2〗were detected, and their frequencies in 39 germplasms were 30.77%, 17.95%, 2.56%, 5.13%, 12.82%, 30.77%, 23.08% and 5.13%, respectively. The germplasms carrying Lr24 and Lr38 showed effective resistance to leaf rust. The germplasms carrying Lr34 or Lr37 alone showed moderately or highly susceptible to leaf rust, respectively, but the germplasms carrying both Lr34 and Lr37 showed increased resistance to leaf rust, reaching moderately resistant degree. Four germplasms (KPL-1, OK.95571, Moscow 39 and Feiheitudi 24) showed outstanding resistance to leaf rust, reaching 0; degree, which were important sources for leaf rust resistance breeding. KPL-1 and OK.95571 carry three and five resistance genes, respectively, but Moscow 39 and Feiheitudi 24 only carry Lr1. We speculated that these two materials contained other undetected rust resistance genes. In conclusion, pyramiding more leaf rust resistance genes is conducive to improve leaf rust resistance.

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