欢迎访问《麦类作物学报》编辑部网站

作者	单位
杜莹莹，顾正中，周羊梅，王安邦，杨子博，张勇	江苏徐淮地区淮阴农业科学研究所/淮安市农业生物技术重点实验室，江苏淮安 223001

摘要点击次数: 241

全文下载次数: 426

中文摘要:

为了解江苏淮北麦区小麦品种（系）重要性状功能基因的组成，利用高通量KASP标记技术对江苏淮北麦区74份小麦品种（系）的产量、品质、抗病虫性以及抗穗发芽相关基因进行检测。结果表明，产量性状相关基因中， TaSus1-7B、 TaSus2-2A、 TaGS3-D1、 TGW6-A1、 TaGW2-6A、 TaCwi-A1、 TaCWI-4A和 TaCWI-5D的高粒重等位变异占供试材料的60%以上，分布频率分别为94.59%、75.68%、90.54%、100%、 98.65%、86.49%、82.43%和100%。品质性状相关基因中，高分子量麦谷蛋白（HWM-GS）基因 Glu-A1、 Glu-B1和 Glu-D1的优异亚基分布频率分别为82.43%、2.70%和47.30%；非1B/1R易位的分布频率为31.08%；非糯质等位变异 Wx-B1a在所有供试材料中均能检测到，而高蛋白质含量等位变异 Gpc-B1（+）在供试材料中均未检测到；籽粒硬度基因 Pina-D1、 Pinb-D1和 Pinb2-V2的硬度等位变异分布频率分别为1.35%、63.51%和25.68%；多酚氧化酶（PPO）活性基因 TaPpo2-2D的低活性等位变异分布频率为18.92%，而 TaPpo2-2A低活性等位变异在供试材料中均未检测到；黄色素含量基因 TaPsy-A1、 TaPsy-B1、 TaPsy-D1、 TaPds-B1、 TaLyc-B1、 TaZds-A1和 TaZds-D1的低黄色素含量等位变异分布频率分别为36.49%、62.16%、93.24%、44.59%、83.78%、16.20%和100%。抗赤霉病基因中，苏麦3号 Fhb1基因型和UMN10 Fhb1基因型的分布频率分别为4.05%和2.70%；抗条锈病基因 Yr15的分布频率为4.05%；抗叶锈病基因 Lr14a和Lr68的分布频率分别为27.03%和40.54%；抗禾谷孢囊线虫病基因 Cre8的分布频率为39.19%。抗穗发芽基因中， TaPHS1、 TaMFT-A1、 TaVP1-B1和 TaSdr-B1的抗穗发芽等位变异的分布频率分别为67.57%、31.08%、67.57%和12.16%。

英文摘要:

In order to understand the composition of functional genes related to important traits of wheat varieties(lines) in Huaibei wheat area of Jiangsu Province,high-throughput KASP marker technology was used to analyze the genes related to yield,quality,disease and insect resistance,and pre-harvest sprouting of 74 wheat varieties(lines). The results showed that the high grain weight allelic variation of TaSus1-7B, TaSus2-2A, TaGS3-D1, TGW6-A1, TaGW2-6A, TaCwi-A1, TaCWI-4A and TaCWI-5D accounted for more than 60% of the tested materials,and the distribution frequencies were 94.59%,75.68%,90.54%,100%,98.65%,86.49%,82.43% and 100%,respectively. The distribution frequency of high-molecular-weight glutenin(HWM-GS) genes Glu-A1, Glu-B1 and Glu-D1 were 82.43%,2.70% and 47.30%,respectively. The distribution frequency of non-1B/1R translocation was 31.08%. The non-waxy allelic variation Wx-B1a was detected in all tested materials,while the high protein content allelic variation Gpc-B1 was not detected in the tested materials. The genotypic frequencies of grain hardness-related genes Pina-D1, Pinb-D1 and Pinb2-V2 were 1.35%, 63.51% and 25.68%,respectively.The frequency of low activity allelic variation of polyphenol oxidase gene TaPpo2-2D was 18.92%,while the low activity types of TaPpo2-2A was not detected.The frequencies of allelic variation of yellow pigment content genes TaPsy-A1, TaPsy-B1, TaPsy-D1, TaPds-B1, TaLyc-B1, TaZds-A1 and TaZds-D1 were 36.49%,62.16%,93.24%,44.59%,83.78%,16.2% and 100%,respectively. Among the Fusarium head blight resistance genes,the frequencies of Sumai 3 Fhb1 and UMN10 Fhb1 were 4.05% and 2.70%,respectively.The stripe rust gene Yr15 counted for 4.05%,and the leaf rust genes Lr14a and Lr68 counted for 27.03% and 40.54%,respectively. The cereal cyst nematode gene Cre8 counted for 39.19%. Among the pre-harvest sprouting resistance genes,the frequencies of TaPHS1, TaMFT-A1, TaVP1-B1 and TaSdr-B1 were 67.57%,31.08%, 67.57% and 12.16%,respectively.

查看全文查看/发表评论下载PDF阅读器

关闭