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作者	单位
王沙沙，黄绍敏，张珂珂，宋晓	1. 河南省农业科学院小麦研究所/河南省小麦生物学重点实验室，河南郑州 450002；2.河南省农业科学院植物营养与资源环境研究所，河南郑州 450002；3.河南省农业生态环境重点实验室，河南郑州 450002

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中文摘要:

为解析小麦硝酸盐转运蛋白基因 TaNRT1.1的生物学功能，本研究通过同源克隆的方法从普通小麦中克隆了小麦硝酸盐转运蛋白基因 TaNRT1.1(TaNRT1.1-1A、 TaNRT1.1-1B和 TaNRT1.1-1D)。生物信息学分析表明，这三个同源基因编码的蛋白均为疏水蛋白，含有丰富的α-螺旋和无未见则卷曲，主要定位于质膜上。小麦不同组织qRT-PCR分析表明， TaNRT1.1-1A和 TaNRT1.1-1B基因在根中表达量最高，其次是叶和茎， TaNRT1.1-1D基因在茎中表达量最高，其次是叶和根。因此，推测 TaNRT1.1-1A和 TaNRT1.1-1B基因在硝酸盐吸收过程中发挥了重要作用， TaNRT1.1-1D基因在硝酸盐转运过程中发挥了重要作用。通过对小麦 TaNRT1.1基因多态性筛选发现，在 TaNRT1.1-1A基因启动子上游1 120 bp的位置有一个8 bp(TGCATGCA)的插入位点，该位点可能与小麦氮利用效率相关。不同氮利用效率小麦品种qRT-PCR分析结果表明，氮高效小麦品种（基因型为 TaNRT1.1-1A-b）苗期根中 TaNRT1.1-1A基因的相对表达量显著高于氮低效小麦品种（基因型为 TaNRT1.1-1A-a）。

英文摘要:

In order to analyze the biological function of the wheat nitrate transporter/peptide transporter gene TaNRT1.1,the TaNRT1.1-1A, TaNRT1.1-1B and TaNRT1.1-1D genes were successfully cloned from bread wheat.Bioinformatics analysis showed that the proteins encoded by the TaNRT1.1 homoeologys were all hydrophobic proteins,containing abundant α-helices and random coil,were mainly localized in plasma membrane.Relative expression levels of different tissues showed that relative expression of TaNRT1.1-1A and TaNRT1.1-1B were highest in roots,followed by that in leaves and stems,and relative expression of TaNRT1.1-1D gene was highest in stems,followed by that in leaves and roots. Therefore,it was speculated that TaNRT1.1-1A and TaNRT1.1-1B genes played an important role in the nitrate uptake process,and TaNRT1.1-1D gene played an important role in nitrate transport process. By screening the polymorphism of the wheat TaNRT1.1 gene,an 8 bp(TGCATGCA) insertion was found in position -1 120 bp of the promoter of TaNRT1.1-1A gene,which was probably associated with nitrogen utilization efficiency in wheat. Relative expression of TaNRT1.1-1A indicated that relative expression levels of wheat cultivars with notrogen-high-efficiency( TaNRT1.1-1A-b allele) was significantly higher than that of wheat cultivars with nitrogen-low-efficiency ( TaNRT1.1-1A-a allele) in seedling root.

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