| The ubiquitin receptor DA1 plays an important role in controlling the size of seeds and organs. In this study,the DA1 gene was identified from the whole wheat genome of wheat Chinese Spring and analyzed by bioinformatics. A total of 12 wheat DA1 homologous genes were identified in the wheat genome,which were located on chromosomes 2B,2D,4A,4B,4D,5A,5B and 5D. The subcellular prediction was performed that they were localized in nucleus. According to phylogenetic analysis, TaDA1s were divided into three categories: TaDA1, TaDAR1 and TaDAR2.Except for the TaDA1-2D1 protein only containing the DA1-like domain,the other members contained DA1-like,UIM and LIM domains.Sequence analysis showed that the promoter region of TaDA1s contained known motifs related to hormones and environmental response,and growth and development.Through public transcriptomic data and qRT-PCR experiments,it was shown that TaDA1s genes were expressed among different tissues. Interestingly,some TaDA1s showed different expression patterns during grain development. ABA could inhibit the expression of TaDA1s.In addition,they were also responsive to low temperature and salt treatment. It was shown that TaDA1-2B,TaDAR1-5A and TaGW2 has transcriptional auto-activation by yeast two hybrid system.Further,luciferase complementation experiment was employed to show there was weak interaction between TaDAR1-5A and TaGW2. In summary,our results lay a foundation for further exploring the biological functions of the DA1 gene family in wheat. |
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