| Lipoxygenase(Lox) in wheat grains is one of the important factors affecting the color of wheat flour. In order to understand the distribution of alleles and Lox activity in wheat varieties from Huanghuai southern region, and clarify the relationship between different alleles and Lox activity.In this study, a total of 94 wheat varieties newly cultivated in the Huanghuai southern region were used as the test materials,the functional markers of Lox16, Lox18 and Lox-B23 were used to detect TaLox-B1, TaLox-B2 and TaLox-B3 loci, and spectrophotometer were used to measure the Lox activity of the test materials. At the TaLox-B1 locus, TaLox-B1a and TaLox-B1b alleles were detected, and their distribution frequencies were 18.1% and 81.9%, respectively. The Lox activity of TaLox-B1a allele was significantly higher than that of TaLox-B1b allele. At the TaLox-B2 locus, TaLox-B2a and TaLox-B2b alleles were detected, and their distribution frequencies were 88.3% and 11.7%, respectively. The Lox activity of TaLox-B2a allele was significantly higher than that of TaLox-B2b allele. At the TaLox-B3 locus, TaLox-B3a and TaLox-B3b alleles were detected, and their distribution frequencies were 67.0% and 33.0%, respectively. The Lox activity of TaLox-B3a allele was significantly higher than that of TaLox-B3b allele. Six allele combinations of Lox activity genes( TaLox-B1a/TaLox-B2a/TaLox-B3a , TaLox-B1b/TaLox-B2a/TaLox-B3a , TaLox-B1a/TaLox-B2a/TaLox-B3b , TaLox-B1b/TaLox-B2a/TaLox-B3b, TaLox-B1a/ TaLox-B2b/ TaLox-B3b and TaLox-B1b/ TaLox-B2b/ TaLox-B3b) were detected with 10.6%, 56.4%, 5.3%, 16.0%, 2.1% and 9.6%, respectively. The Lox activity of the allele combination TaLox-B1a/ TaLox-B2a/ TaLox-B3a was significantly higher than the other five allele combinations,and the Lox activity of the allele combination TaLox-B1b/ TaLox-B2b/ TaLox-B3b was significantly lower than the other five allele combinations. |
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