| In order to effectively utilize the introduced wheat germplasm resources, clarify the composition and distribution of dwarfing genes and determine the effect of height reduction, molecular detection of dwarfing genes was carried out in 47 wheat germplasms introduced from Chile, the United States, Mexico and Australia using eight specific molecular markers associated with dwarf genes Rht-B1b, Rht-D1b, Rht4, Rht5, Rht8, Rht9, Rht12 and Rht13. The results showed that 46 of the 47 wheat germplasms have dwarf genes, among which Rht-B1b, Rht4 and Rht12 had the highest frequencies (40.43%each), followed by Rht8(31.91%), Rht-D1b and Rht13(19.15% each), Rht5 (8.51%), and Rht9(6.38%). There were 10 germplasms with single dwarf gene, accounting for 21.28%; 36 germplasms with two or more dwarf genes, accounting for 76.60%. Among them, 11 germplasms contain 3 dwarf genes and 2 germplasms contain 4 dwarf genes. In the germplasm population, Rht5 had the highest effect on height reduction, with an average plant height of 86.65 cm in all the wheats carrying Rht5. The average plant height of wheat with desirable gene Rht9, Rht12 and Rht13 was 101.07 cm,92.87 cm and 92.75 cm, respectively,and Rht9 had the weakest effect in 8 dwarf genes. |
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