| In order to further clarify the relationship between high-molecular-weight glutenin subunits(HMW-GS) and wheat quality traits, a total of 127 wheat cultivars or advanced lines in Huanghuai wheat region were used to identify HMW-GS subunits and determine the quality by the technologies of SDS-PAGE, near infrared grain analyzer,farinograph and extensograph.Results showed that the tested materials had abundant polymorphisms at the three gene loci of Glu-A1, Glu-B1 and Glu-D1, of which, two(Ax1, Ax-null), four(Bx7+By8, Bx7+By9, Bx14+y15, Bx17+By18), and two(Dx5+Dy10, Dx2+Dy12) types of subunits were detected, respectively. The highest frequency of allele was Ax1, Bx7+By9 and Dx5+Dy10 at each of the three loci, which accounted for 70.1%, 42.5% and 51.2%, respectively. A total of 14 HMW-GS combinations were identified in the tested materials, among which the combinations of 1Ax1/1Bx7+1By8/1Dx5+1Dy10 and 1Ax1/1Bx7+1By9/1Dx2+1Dy12 had higher frequency, which accounted for 18.9% and 17.3%, respectively. For single locus, subunits 1Ax1, 1Bx7+1By8, 1Bx17+1By18, 1Dx5+1Dy10 had significantly positive effects on protein, Zeleny value, stability, max resistance and stretch area, while subunit 1Bx14 + 1By15 had negative effects on other quality traits except protein and wet gluten content. At the same time, it was found that the varieties(lines) with the combination of 1Ax1/1Bx7+1By8/1Dx5+1Dy10 showed significantly greater quality traits and comprehensively outstanding quality than those with other combinations, followed by varieties(lines) with the combination of 1Ax1/1Bx17+1By18/1Dx5+1Dy10. However, the quality traits of the varieties(lines) with the combinations of 1Ax-null/1Bx7+1By9/1Dx2+1Dy12 and 1Ax-null/1Bx14+1By15/1Dx5+1Dy10 were significantly lower than those with other combinations. The results can provide reference for further improving the utilization rate of good quality subunits and genetic improvement of wheat quality in China. |
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