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李兴茂,王淑英, 倪胜利.小麦旗叶与幼苗性状的QTL分析[J].麦类作物学报,2021,(5):532
小麦旗叶与幼苗性状的QTL分析
QTL Mapping for Traits of Flag Leaf and Seedling in Wheat
  
DOI:10.7606/j.issn.1009-1041.2021.05.02
中文关键词:  幼苗期  旗叶  数量性状位点
英文关键词:Seedling stage  Flag leaf  QTL
基金项目:国家自然科学基金项目(31460349)
作者单位
李兴茂,王淑英, 倪胜利 (甘肃省农业科学院旱地农业研究所甘肃兰州 730070) 
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中文摘要:
      为挖掘控制小麦幼苗性状与旗叶性状的QTL,并探讨两者的遗传基础,以京冬8号和矮抗58构建的RIL群体(207个家系)为材料,田间试验测定旗叶相关性状,水培试验测定幼苗期相关性状,通过完备区间作图对这些性状进行QTL研究。结果共检测到10个控制旗叶性状的QTL,单个QTL可解释1.98%~ 9.89%的表型变异,其中有6个QTL为主效QTL,分别位于1A、4D和5D染色体上;共检测到22个控制幼苗性状的QTL,单个QTL可解释1.14%~10.52%的表型变异,仅有2个QTL为主效QTL,分别位于1A和4D染色体上。除3D染色体上控制幼苗根长的QTL以及5D染色体上控制旗叶面积和旗叶宽的QTL表现为部分显性效应外,与其他性状有关的QTL均表现为超显性效应。1A、2D、4D、5A、5D和7A染色体上的分子标记存在多效性,其中2D(wmc170)和4D(barc308)染色体上与幼苗性状QTL紧密连锁的分子标记(wmc170和barc308)也与旗叶性状QTL紧密连锁。
英文摘要:
      In order to dissect the genetic basis of seedling traits and flag leaf traits by mapping quantitative trait loci(QTLs) of target traits,RIL population consisting of 207 lines constructed by Jingdong 8 and Aikang 58 was used as materials,The flag leaf traits of RIL populations and their parents were collected in the field,and the seedling traits were determined by hydroponic test.QTLs for these traits were studied by inclusive composite interval mapping(ICIM).The results showed that a total of 10 QTLs controlling flag leaf traits were detected,each QTL explained 1.98%-9.89% of phenotypic variance,and 6 major QTLs were mapped on chromosomes 1A,4D and 5D; 22 QTLs controlling seedling traits were detected,each QTL explained 1.14%-10.52% of phenotypic variance,and 2 major QTLs were mapped on chromosomes 1A and 4D.Expect QTL controlling seedling length on chromosome 3D and QTLs controlling flag leaf area and flag leaf width on chromosome 5D showed partial dominant effect,all the other QTLs showed over-dominant effect.There were pleiotropic molecular markers on chromosomes 1A,2D,4D,5A,5D and 7A,respectively,and the molecular markers(wmc170 and barc308) which tightly linked to QTLs controlling seedling traits on chromosomes 2D and 4D also tightly linked to QTLs controlling flag leaf traits.
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