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作者	单位
曹志琛，吴娴，汪德州，柳珊，杨慧玉，郝小聪，房兆峰，朱文根，王伟伟，王小燕，唐益苗	(1.长江大学农学院，湖北荆州 434025； 2.北京市农林科学院杂交小麦工程技术研究中心，北京 100097)

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中文摘要:

多蛋白桥梁因子（multiprotein bridging factor 1,MBF1）是一类广泛存在于植物中的转录共激活因子，在植物生长发育和逆境响应过程中发挥重要作用。为进一步了解该蛋白的功能，利用同源克隆法从小麦中获得 MBF1基因，根据其染色体位置分别命名为 TaMBF1c A、 TaMBF1c B和 TaMBF1c D，通过生物信息学方法分析其蛋白序列特征、蛋白三级结构及顺式作用元件，并利用RT qPCR技术分析其组织表达特异性以及干旱、热胁迫响应模式。序列分析表明，TaMBF1c A、TaMBF1c B和TaMBF1c D分别含有456、471和465 bp的开放阅读框；三维结构预测发现，TaMBF1c A、TaMBF1c B和TaMBF1c D在N端和C端分别含有MBF1结构域和4个α螺旋组成的helix turn helix结构域；系统进化分析结果表明， TaMBF1c基因与二粒小麦（Triticum dicoccum）的亲缘关系最近。顺式作用元件分析发现， TaMBF1c A、 TaMBF1c B和 TaMBF1c D启动子区都含有干旱响应元件（MBS element/MYB element）和热响应元件（HSE element）。RT qPCR分析显示， TaMBF1c A和 TaMBF1c B基因在叶片中特异性表达， TaMBF1c D只在根部特异性表达；在干旱胁迫条件下， TaMBF1c在干旱敏感小麦品种中表达量较高，其表达量是 TaMBF1c在耐旱小麦品种中表达量的280倍， TaMBF1c在干旱信号途径中起负调控作用。在热胁迫条件下， TaMBF1c A和 TaMBF1c B在热敏感与耐热小麦品种中上调表达，而 TaMBF1c D仅在热敏感小麦品种中上调表达，明显高于耐热小麦品种。

英文摘要:

Multiprotein bridging factor 1（MBF1） is a type of transcription co activator that is widely present in plants, and it plays an important role in plant growth and development and stress response process.In order to further understand the function of this protein,the MBF1 gene was obtained from wheat by homologous cloning, named TaMBF1c A, TaMBF1c B and TaMBF1c D according to its chromosomal location. Protein sequence characteristics, protein three dimensional structures and cis acting elements were analyzed by bioinformatics methods, and the gene expression pattern was analyzed using RT qPCR technology under drought and heat stresses. Sequence analysis showed that TaMBF1c A, TaMBF1c B and TaMBF1c D contain open reading frames of 456 bp, 471 bp and 465 bp, respectively;Three dimensional structure prediction revealed that TaMBF1c A,TaMBF1c B and TaMBF1c D contained MBF1(Multiprotein bridging factor 1) and helix turn helix composing of four α helices in N terminal and C terminal,respectively.Phylogenetic analysis results showed that the TaMBF1c gene has the closest genetic relationship with emmer wheat(T.dicoccum). Analysis of cis acting elements found that the promoter regions of TaMBF1c A, TaMBF1c B and TaMBF1c D all contained drought response elements (MBS element/MYB element) and heat response elements (HSE elements). RT qPCR analysis showed that TaMBF1c A and TaMBF1c B genes were specifically expressed in leaves, and TaMBF1c D was specifically expressed only in roots; under drought stress condition, TaMBF1c was highly expressed in drought sensitive wheat, and its expression level was about 280 times of that in drought tolerant wheat, indicating TaMBF1c might have a negative regulatory role in drought signaling pathway. Under heat stress conditions, TaMBF1c A and TaMBF1c B were induced up regulation in heat sensitive and heat tolerant wheat, while TaMBF1c D was up regulated only in heat sensitive wheat,and is significantly higher than that in heat tolerant wheat.

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