| 战帅帅 ，严勇亮，刘鑫源，王丽丽 ，耿洪伟.新疆小麦阿拉伯木聚糖阿魏酸酰基转移酶基因 TaBahd-A1等位变异检测及其分布规律[J].麦类作物学报,2020,(12):1416 |
| 新疆小麦阿拉伯木聚糖阿魏酸酰基转移酶基因 TaBahd-A1等位变异检测及其分布规律 |
| Molecular Identification and Distribution Analysis of TaBahd-A1 Gene in Xinjiang Wheat |
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| DOI：10.7606/j.issn.1009-1041.2020.12.02 |
| 中文关键词: 新疆小麦 阿拉伯木聚糖阿魏酸酰基转移酶基因 等位变异 功能标记 |
| 英文关键词:Xinjiang winter wheat Arabinogas ferulicacid transferase gene Allelic mutation Functional marker〖HJ〗 |
| 基金项目:国家自然科学基金项目（31771786）；新疆自治区天山雪松计划项目（2018XS04）；新疆自治区高校科研计划自然科学重点项目（XJEDU2020I010）；新疆农业大学重点学科项目（XNCDKY2018005） |
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| 中文摘要: |
| 阿魏酰阿拉伯木聚糖（feruloyl arabinoxylan，FAX）含量是影响小麦加工品质的重要因素，揭示其相关基因在小麦中的遗传变异规律，可为小麦分子育种提供一定参考。本研究利用阿拉伯木聚糖阿魏酸酰基转移酶 TaBahd-A1基因位点的3对功能标记AFTA2、AFTB2和AFTC8，对124份新疆小麦品种（系）进行 TaBahd-A1等位变异检测，并分析等位变异分布规律。结果表明，携带与低FAX含量相关的 TaBahd-A1b等位变异材料占比（57.2%）最大，其次是与高FAX含量相关的 TaBahd-A1c等位变异（29.1%），与高FAX含量相关的 TaBahd-A1a等位变异占比（13.7%）最小；其中，98份新疆冬小麦品种（系）中，携带 TaBahd-A1a和 TaBahd-A1c基因型的材料共占比38.7%，携带 TaBahd-A1b基因型的材料占比61.3%；26份新疆春小麦材料中，携带 TaBahd-A1a和 TaBahd-A1c基因型的材料共占比57.6%，携带 TaBahd-A1b基因型的材料占比38.4%。在不同类型冬小麦品种（系）中，与高FAX含量相关的等位变异类型 TaBahd-A1a和 TaBahd-A1c的分布频率表现为引进品种（系）＞地方品种>自育品种（系）；与低FAX含量相关的等位变异类型 TaBahd-A1b的分布频率表现为自育品种（系）＞地方品种＞引进品种（系）。 |
| 英文摘要: |
| Feruloyl arabinoxylan(FAX) content affects wheat processing quality,revealing the genetic variation of related genes in different wheat varieties(lines) in Xinjiang can provide a certain reference for further improvement of wheat breeding in Xinjiang. In this study, 3 pairs of functional markers AFTA2, AFTB2, and AFTC8 were used to detect the allelic variation of the Arabinoxylan feruloyl acyl transferase gene TaBahd-A1 in 124 Xinjiang wheat varieties(lines), and the distribution of allelic variation was analyzed. The results showed that the proportion of 3 allelic variations in total materials ranked as TaBahd-A1b(57.2%), TaBahd-A1c(29.1%) and TaBahd-A1a(13.7%). Among the 98 winter wheat varieties(lines), materials with TaBahd-A1a and TaBahd-A1c associated with high FAX content accounted for 38.7%,materials with TaBahd-A1b associated with low FAX content accounted for 61.3%. Among the 26 Xinjiang spring wheat materials, the proportion of materials with TaBahd-A1a and TaBahd-A1c allele was 57.6%,the proportion of materials with TaBahd-A1b allele was 38.4%. Among different types of Xinjiang winter wheat varieties(lines), the distribution frequencies of materials with high FAX content-related allelic variants TaBahd-A1a and TaBahd-A1c ranked as introduced varieties(lines) ＞landraces>self-bred varieties,the distribution frequency of materials with TaBahd-A1b associated with low FAX content ranked as self-bred varieties(lines) ＞landraces＞introduced varieties. |
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