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作者	单位
王秀华，于鸿翔，潘香逾，赵岩，李斯深	（1.山东农业大学农学院，作物生物学国家重点实验室，山东泰安 271018； 2.山东农业大学资源与环境学院，山东泰安 271018）

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中文摘要:

小麦萌发期对水分和盐胁迫敏感，对该时期水分和盐胁迫下种子萌发性状进行QTL定位具有重要的意义。本研究以小麦“泰农18×临麦6号” RIL群体为材料，以20%PEG-6000溶液和100 mmol·L^-1 NaCl溶液分别模拟水分和盐胁迫环境，对种子萌发期10个性状进行了QTL定位。结果表明，在正常、水分胁迫和盐胁迫3种不同处理下各性状变异较大。相关分析表明，抗旱和耐盐可能是两个独立遗传的性状，胚芽鞘长可以作为节水抗旱的鉴定指标。3种处理下共检测到10个萌发相关性状的103个QTL，其中，17个为相对高频QTL（RHF-QTL），分布在7条染色体（1A、3A、3B、4B、7A、7B和7D）上，平均贡献率为7.55%~15.97%。这些RHF-QTL形成4个QTL簇（QTL cluster,QC），分布在3A、7A和7D染色体上。其中，7A染色体上的QC2包括3个RHF-QTLs（ QSdw-7A.1、 QGf-7A.1和 QGi-7A.1），均与小麦耐盐性相关；7D染色体上的QC3包括2个RHF-QTLs（ QGi-7D.1、 QGdrc-7D.1），均与小麦节水抗旱性相关。这2个QCs增加效应均来自母本泰农18。本研究获得的RHF-QTLs和QCs，可为小麦萌发期节水抗旱和耐盐分子标记辅助选择提供理论和技术支持。

英文摘要:

Germination stage is the initial stage of wheat growth,and it is sensitive to water and salt stress. QTL analysis of wheat seed germination traits under water and salt stresses can obtain important stress tolerance sites at this period.The experiment was carried out using RILs (recombinant inbred lines) derived from the cross of ‘Tainong 18 × Linmai 6’in wheat. Water stress and salt stress were simulated with 20% PEG-6000 solution and 100 mmol·L^-1 NaCl,respectively. QTLs were analyzed for 10 traits of seed germination. The result showed that RIL population had great variation under normal conditions,water stress and salt stress treatments.The correlation analysis indicated that drought resistance and salt tolerance might be two independent genetic traits,and sheath length(SHL) could be used as the identification index for drought resistance.A total of 103 QTLs were detected for the 10 seed germination traits under three treatments. Of all these QTLs,17 relatively high-frequency QTLs (RHF-QTLs) were detected with the average contribution rate ranging from 7.55% to 15.97%,distributing on chromosomes 1A,3A,3B,4B,7A,7B and 7D.4 QTL clusters(QCs) were formed with these RHF-QTLs,distributing on chromosomes 3A,7A and 7D.QC2 located on chromosome 7A was related to salt tolerance in wheat,containing three RHF-QTLs ( QSdw-7A.1, QGf-7A.1 and QGi-7A.1).QC3 located on chromosome 7D was related to drought resistance in wheat,containing two RHF-QTLs ( QGi-7D.1 and QGdrc-7D.1).The increasing effects of these two QCs were derived from female parent Tainong 18(TN18). These two QCs may improve salt tolerance and drought resistance by improving nutrient utilization efficiency. These RHF-QTLs and QCs obtained in this study could provide theoretical and technical support for molecular marker assisted selection for drought resistance and salt tolerance of wheat at the germination stage.

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