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作者	单位
孙淑珍，田中伟，郑巧梅，孙传蛟，孙建云	（1.南京农业大学农学院，农业农村部作物生理生态与生产管理重点实验室，江苏南京 210095； 2.南京农业大学生命科学院，江苏南京 210095）

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中文摘要:

为明确外源调节物质对Cr⁶⁺胁迫下小麦种子萌发的缓解机制，利用水培试验研究了葡萄籽提取物（grape seed extract，GSE）、氯化血红素（hematin chloride，Hemin）和茉莉酸甲酯（methyl jasmonate，MeJA）对Cr⁶⁺胁迫下小麦种子萌发的影响。结果表明，Cr⁶⁺胁迫显著降低了小麦种子发芽率、幼苗根长、株高、干物质积累量及种子α-淀粉酶活性，同时提高了小麦根系渗透调节物质的含量，并引发氧化胁迫。外源GSE、Hemin和MeJA均可有效缓解Cr⁶⁺胁迫对小麦种子萌发的不良影响，促进种子萌发和幼苗生长，提高小麦根系可溶性糖和可溶性蛋白含量，增强α-淀粉酶、超氧化物歧化酶（superoxide dismutase，SOD）、过氧化氢酶（catalase，CAT）、抗坏血酸过氧化物酶（ascorbase peroxidase，APX）和谷胱甘肽还原酶（glutathione reductase，GR）的活性。其中，GSE对Cr⁶⁺胁迫的缓解效果最佳。总之，外源调节物质可以通过提高Cr⁶⁺胁迫下小麦种子萌发过程中的淀粉水解能力、渗透调节能力和抗氧化能力，提高小麦的耐Cr⁶⁺性。

英文摘要:

In order to clarify the alleviation mechanism of exogenous regulators on wheat seed germination under Cr⁶⁺stress,the effect of grape seed extract(GSE),hematin chloride(Hemin) and methyl jasmonate(MeJA) on wheat seed germination under Cr⁶⁺ stress were studied by hydroponic.The results showed that Cr⁶⁺ stress significantly reduced wheat seed germination rate,plant root length,shoot height,dry weight and α-amylase activity during wheat germination,simultaneously increased the content of osmotic regulators in wheat roots and induced oxidative stress. GSE,Hemin and MeJA effectively alleviated Cr⁶⁺stress,which promoted seed germination and growth,increased the contents of soluble sugar and soluble protein in wheat root,and improved the activities of α-amylase,superoxide dismutase(SOD),catalase(CAT),ascorbase peroxidase(APX) and glutathione reductase(GR). Among them,GSE has the best alleviation effect on Cr⁶⁺ stress.In conclusion,exogenous regulators can improve Cr⁶⁺ tolerance by improving amylolysis,osmotic adjustment and oxidation resistance abilities during the germination of wheat seeds under Cr⁶⁺ stress.

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