| The wheat cv. Xinong 979 has good resistance to FHB. In order to explore FHB disease resistance genes in Xinong 979, Xinong 979 and a susceptible wheat cv. Zhoumai 18 were inoculated by Fusarium graminearum strain BN-8. The glumes of the two cultivars at 12, 24 and 96 h after inoculation and the glumes of Xinong 979 before inoculation were collected for transcriptome sequencing to screen differentially expressed genes(DEGs) related to FHB resistance.GO function annotation and KEGG pathway analysis were carried out for the DEGs. The results showed that there were 2 122 DEGs, including 14, 1 978 and 142 DEGs at 12, 24 and 96 h after inoculation, respectively. GO function annotation classified these DEGs into 43 functional groups of three major classes, mainly involving metabolic process, cellular process, response to stimulus, biological regulation,cell part,cell,catalytic activity,binding and transporter activity. KEGG pathway analysis showed that protein processing in endoplasmic reticulum enriched the most DEGs(48), followed by photosynthesis-antenna proteins, containing 41 DEGs and 30 DEGs annotated in starch and sucrose metabolism, respectively. The pathways significantly enriched were photosynthesis-antenna proteins, protein processing in endoplasmic reticulum, and terpenoid backbone biosynthesis, etc. There were 27 DEGs participated in plant hormone signal transduction, involved in abscisic acid pathway, jasmonic acid pathway and so on.19 DEGs were found in plant-pathogen interaction, encoding calcium-binding proteins, WRKY transcription factors, disease resistance proteins, and so on. In addition, we also screened detoxification related protein genes, such as UDP-glucosyltransferase genes. |
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