| To determine the effects of different nitrogen(N) regulation on wheat N assimilation and yield, five different treatments were carried out by using Wanmai 68 as the material, including traditional fertilization+biochar(CN+C),traditional fertilization+ nitrification inhibitor(DMPP)(CN+D),traditional fertilization+ foliar fertilizer(CN+P), no N fertilizer(CK) and traditional fertilization(CN). The results showed that compared with CN, application of biochar and nitrification inhibitor improved the content of ammonium nitrogen, nitrate reductase(NR) and glutamine synthetase(GS) activity in wheat,and foliar spraying improved the content of ammonium nitrogen and NR activity during wheat filling period. Compared with CN, N regulations increased not only the content of free amino acid and soluble protein in wheat filling period but also the N transport rate and N metabolism in wheat, and improved N use efficiency, agronomic N use efficiency and partial factor productivity of applied N, thus improved the wheat yield. Among them, biochar, nitrification inhibitor and foliar fertilizer were applied to increase 568.3 kg·hm-2, 520.0 kg·hm-2 and 663.3 kg·hm-2, respectively. |
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