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胡文静,梁秀梅,高致富,高德荣,程顺和.扬麦17/宁麦18的F2群体株高QTL定位及分析[J].麦类作物学报,2019,(7):768
扬麦17/宁麦18的F2群体株高QTL定位及分析
QTL Mapping for Plant Height Using the F2 Population of Yangmai 17/Ningmai 18
  
DOI:10.7606/j.issn.1009-1041.2019.07.02
中文关键词:  小麦  株高  分子标记  QTL
英文关键词:Wheat  Plant height  Molecular marker  QTL
基金项目:国家重点研发计划项目(2016YFD0101802,2017YFD0100801);江苏省自然科学基金项目(BK20171279);国家现代农业产业技术体系建设专项(CARS-3-2-11);扬州市现代农业项目(YZ2016033)
作者单位
胡文静,梁秀梅,高致富,高德荣,程顺和 (1.江苏里下河地区农业科学研究所/农业农村部长江中下游小麦生物学与遗传育种重点实验室江苏扬州 2250072.河南农业大学/河南粮食作物协同创新中心河南郑州 450002) 
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中文摘要:
      扬麦17和宁麦18均为高产小麦品种,为研究两者的株高遗传机制,以扬麦17与宁麦18杂交获得的310个F2单株及其衍生F2:3家系为材料,利用已构建的覆盖19条染色体的遗传连锁图谱,对株高性状进行QTL定位。应用复合区间作图法定位到8个株高QTL,分别位于2D、4B、5A、5B和7A染色体上,4个QTL在两世代中均检测到,其中 QPh-YN-5A.1为一主效株高QTL位点,矮秆效应来自扬麦17,LOD值为8.69和12.81,可解释14.36%和20.46%的表型变异; QPh-YN-2D.1 QPh-YN-5B.2 QPh-YN-7A的矮秆效应来自宁麦18,可在两世代中分别解释2.84%和6.37%、4.26%和5.46%、2.15%和3.16%的表型变异。另外4个QTL仅在单世代检测到,其中 QPh-YN-2D.2 QPh-YN-4B QPh-YN-5B.1的矮秆效应来自宁麦18,可分别解释 5.05%、11.24%和4.62%的表型变异, QPh-YN-5A.2的矮秆效应来自扬麦17,可解释 7.72%的表型变异。
英文摘要:
      Both Yangmai 17 and Ningmai 18 are high-yielding wheat varieties. To study the genetic mechanism of plant height,the population of 310 F2 and its F2:3 generations derived from the hybridization between Yangmai 17 and Ningmai 18 were used to detect the quantitative trait loci(QTLs) for plant height. A genetic linkage map covering 19 chromosomes of wheat was established,and QTL mapping of plant height traits was carried out. A total of eight plant height QTLs were mapped by Composite Interval Mapping(CIM) based on the genetic map and phenotypic data,which are located on 2D,4B,5A,5B and 7A chromosomes,and four QTLs were detected in both generations. QPh-YN-5A.1 is a main-effect QTL,explaining 14.36% and 20.46% of the phenotypic variances,the dwarfing effect of which was contributed by Yangmai 17. QPh-YN-2D.1, QPh-YN-5B.2 and QPh-YN-7A explained 2.84% and 6.37%,4.26% and 5.46%,2.15% and 3.16% of the phenotypic variances respectively,the dwarfing effect of which was contributed by Ningmai 18. Four QTLs were detected only in a single generation. QPh-YN-2D.2, QPh-YN-4B and QPh-YN-5B.1 explained 5.05%,11.24% and 4.62% of the phenotypic variances respectively,the dwarfing effect of which was contributed by Ningmai 18. QPh-YN-5A.2 explained 7.72% of the phenotypic variances,the dwarfing effect of which was contributed by Yangmai 17.
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