欢迎访问《麦类作物学报》编辑部网站

作者	单位
李文静，赵新颖，纪萌琦，郭鑫，郭红，邓志英，田纪春	（山东农业大学农学院/作物生物学国家重点实验室/小麦品质育种研究室/山东小麦玉米周年高产高效生产协同创新中心，山东泰安 271018）

摘要点击次数: 846

全文下载次数: 613

中文摘要:

抽穗期是与小麦适应性直接相关的一个很重要的性状，直接或间接影响小麦的产量和抗性。为明确控制抽穗期的关键表达基因，以抽穗期分别为196 d和184 d的小麦近等基因系Y57和96-2为材料，对其二棱期幼穗分别构建转录组测序文库，利用Illumina HiSeq 2500平台进行RNA-seq测序；将获得的clean read与中国春参考序列比对，得到unique read，采用FPKM法计算基因表达量，对差异表达基因进行功能注释、GO和COG功能分类以及KEGG通路分析。结果表明，供试材料经过测序获得质量不低于30的碱基比例(Q30)均高于85.5%；Y57和96-2分别有60 246 194和60 963 580条clean read，其中63.11%和69.51%能与参考基因组序列匹配。共筛选到395个差异表达基因，有382个基因得到功能注释；有298个基因获得GO功能注释，主要涉及细胞组分、结合、细胞反应和代谢过程；COG注释的基因主要涉及一般功能、信号转导机制和转录；KEGG功能注释分析发现，显著富集在光合作用-天线蛋白通路上的基因均上调表达。编码丝氨酸/苏氨酸蛋白磷酸酶2A的基因在Y57中几乎不表达，而在96-2中表达量较高。进一步分析发现，多个与生长发育、抽穗开花相关的转录因子在两个材料中的表达差异显著。此结果可为深入研究抽穗期相关基因和穗发育调控机制奠定基础。

英文摘要:

Heading date is one of the most important traits directly relating to the wheat adaptability and affects the wheat yield and resistance traits. Therefore,it is of significance for breeding and improving the wheat varieties with high yield at molecular level by mining heading-related genes. As the double-ridge stage is the key period affecting the heading date,the RNA of immature spikes at the double-ridge stage from two near-isogenic lines Y57(196 days) and 96-2(184 days) with different heading date were used to construct the transcriptome libraries and sequenced by Illumina HiSeqTM 2500 platform in order to identify the important differentially expressed genes(DEGs) for heading date. RNA-seq clean reads were aligned to the Chinese Spring genome sequences to obtain unique reads. Gene expression level was counted by computing the FPKM value. The functions and pathways of these DEGs were annotated by searching against the GO,COG and KEGG public database. The results indicated that Q30 is over 85.5%. Clean reads from Y57 and 96-2 were 60 246 194 and 60 963 580,respectively. There were 63.11% and 69.51% clean reads mapped to the reference genome sequences,respectively. Three hundred and ninety five DEGs were identified after differential expression analysis. Among them,382 DEGs were annotated in Nr database,and 298 genes were annotated by GO functional enrichment analysis,which are primarily involved in cell part,binding,cellular process and metabolic process. COG analysis showed that DEGs are mainly involved in general function,signal transduction mechanisms and transcription.Through KEGG analysis,we found all DEGs enriched in photosynthesis-antenna protein pathway were up-regulated. Both genes encoding serine/threonine protein phosphatase 2A were highly expressed in 96-2 but lowly expressed in Y57. Further analysis found that the expression level of many transcription factors associated with development and flowering were significantly different. These results would provide abundant resources for further studies on genes related to heading date and laid a foundation for molecular mechanisms.

查看全文查看/发表评论下载PDF阅读器

关闭