| Wheat soil-borne mosaic virus disease is one of the most important problems threating winter wheat. The causal pathogen of the disease includes multi-species of virus, of which wheat yellow mosaic virus(WYMV) and Chinese wheat mosaic virus(CWMV) are major. It was spread by Pofymyxagraminis L. in a persistent manner. In order to determine current status of distribution of the disease, based on field surveys, suspected diseased wheat samples were collected from Shandong province from 2013 to 2016 and detected by dot-ELISA(dot-Enzyme linked immunosorbent assay) and RT-PCR. The results showed that wheat soil-borne virus disease mainly distributed in the south of Shandong province but sporadically occurred in the center, the east and the north of Shandong province. WYMV was detected in samples collected from Linyi, Jining, Taian and Zaozhuang. CWMV were detected in samples collected from Yantai, Weihai, Qingdao, Linyi and Dezhou. Both WYMV and CWMV were detected in samples collected from Yantai, Weihai, Qingdao and Linyi. We analysed the coat protein(CP) gene sequence of CWMV, and constructed the phylogenetic tree. The results indicated that homology of CP gene of CWMV strains was from 94.7% to 98.3%, of which strains sampled from Pingyuan, Wendeng, Fushan and Junan had a closer phylogenetic relationship with that of Japan(accession no.AB299272.1). Strains sampled from Yishui, Rongcheng and Jiaozhou represented a closer relationship with that of Jiangsu(accession no.EF121374.1). Our results, in the present study, provided reference for the reasonable layout of wheat variety cultivation. |
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