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杨漫宇,杨足君,杨武云,杨恩年.多重易位小麦新品种(系)的选育及细胞学鉴定[J].麦类作物学报,2018,(2):127
多重易位小麦新品种(系)的选育及细胞学鉴定
Development and Identification of New Wheat Varieties(Lines) with Multiple Translocation Chromosomes via Cytogenetic Method
  
DOI:10.7606/j.issn.1009-1041.2018.02.01
中文关键词:  小麦  多重易位系  育种
英文关键词:Wheat  Multiple translocation lines  Breeding
基金项目:四川省科技计划项目(应用基础计划类型)(2016JY0070);国家重点研发计划项目(2017YFD0100900)
作者单位
杨漫宇,杨足君,杨武云,杨恩年 (1.四川省农业科学院作物研究所四川成都 610066 2.电子科技大学生命科学与技术学院四川成都 611731) 
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中文摘要:
      为了进一步研究多重染色体易位在培育小麦新品种(系)中的潜在价值,对四川省农业科学院作物研究所育成的小麦品种川麦62进行了荧光原位杂交分析,发现川麦62含5BS·7BS和5BL·7BL易位染色体。从杂交组合内麦8号/2*川麦62的后代中选育出了农艺性状优异的高代稳定品系16EW381和16EW458,原位杂交鉴定发现16EW458是含6VS·6AL、5BS·7BS和5BL·7BL的三重易位系,16EW381是含6VS·6AL、5BS·7BS、5BL·7BL、3BS·5AS和3BL·5AL的五重易位系。同时,产量比较和抗病性鉴定结果表明,16EW381和16EW458产量及对条锈病和白粉病的抗性有明显提高。综上,本研究将为今后的小麦育种及种质创新提供一个新的思路。
英文摘要:
      In order to reveal the potential value of multiple chromosome translocations in wheat breeding program, fluorescence in situ hybridization(FISH) was used to analyze the wheat varieties Chuanmai 62, which was developed by Crop Research Institute of Sichuan Academy of Agricultural Sciences. It was found that Chuanmai 62 contains 5BS·7BS and 5BL·7BL translocation chromosomes. In the progeny derived from the cross combination Neimai 8/2*Chuanmai 62, two elite wheat lines 16EW381 and 16EW458 were obtained. FISH analysis of the two wheat lines indicated that line 16EW458 was a triple translocations line containing 6VS·6AL, 5BS·7BS and 5BL·7BL translocation chromosomes, and 16EW381 was a multiple chromosome translocations line containing 6VS·6AL, 5BS·7BS, 5BL·7BL, 3BS·5AS and 3BL·5AL translocation chromosomes.Meanwhile,yield comparison and resistance identification of 16EW381 and 16EW458 indicated that the yield and resistance of the two wheat lines had been improved significantly.In a conclusion,this study will provide a new opinion for wheat breeding program and wheat germplasm innovation.
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