| To understand the regulative effect of H2O2, a signaling molecule on the physiological response of oat (Avena nuda L.) seedlings under alkali stress, in the present study, a sand culture experiment was conducted to investigate the effect of H2O2 on leaves active oxygen metabolism, osmotica accumulation and growth of ‘Dingyou 6’ oat seedlings under 100 mmol·L-1 NaHCO3 stress. The results showed that under the NaHCO3 stress, the application of 50 and 150 μmol·L-1 H2O2 both could effectively decrease the contents of superoxide anion, H2O2, malondialdehyde, soluble sugar and plasma membrane permeability in leaves, while increased the contents of soluble protein, proline, carotenoid and chlorophyll a/b values. The application of 50 μmol·L-1 H2O2 significantly alleviated the inhibitory effect of NaHCO3 stress on the growth of oat seedlings, decreased superoxide dismutase (SOD) activity and glutathione (GSH) content, and increased activities of catalase (CAT), peroxidase (POD), ascorbate peroxidase (APX) and ascorbic acid (AsA) content in the leaves of the seedlings under NaHCO3 stress. While 150 μmol·L-1 H2O2 had no significantly alleviate growth inhibitory of oat seedlings induced by NaHCO3 stress, but it increased SOD, POD, APX activities and AsA content, and decreased CAT activity, but no significant difference was observed in the GSH content compared with NaHCO3 stress. |
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