刘 洋,赵继新,谈宏斌,王秀娟,李家创,梁邦平,刁慧珊,白宇皓,武 军,陈新宏.小麦-滨麦2D/ 2Ns异代换系DM2411的分子细胞遗传学鉴定[J].麦类作物学报,2017,(5):571 |
小麦-滨麦2D/ 2Ns异代换系DM2411的分子细胞遗传学鉴定 |
Molecular Cytogenetic and Morphological Identification of a Wheat-Leymus mollis 2Ns (2D) Substitution Line DM2411 |
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DOI:10.7606/j.issn.1009-1041.2017.05.01 |
中文关键词: 小麦 滨麦 代换系 GISH EST-STS SSR |
英文关键词:Wheat L.mollis Substitution line GISH EST-STS SSR |
基金项目:陕西省自然科学基金项目(2015JM3095);西北农林科技大学唐仲英育种基金项目 |
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中文摘要: |
为进一步挖掘利用滨麦优异基因,并丰富小麦遗传种质资源,利用形态学、细胞遗传学、基因组原位杂交(Genomic in situ hybridization,GISH)、EST-STS分子标记、SSR分子标记等技术,对从八倍体小滨麦M842-16和硬粒小麦D4286杂交F7代材料中筛选出的1个遗传稳定的小滨麦异代换系DM2411进行了鉴定。细胞遗传学观察表明,DM2411的染色体主要构型为2n=42=21Ⅱ,遗传稳定。根尖体细胞和花粉母细胞的原位杂交研究表明,DM2411含有1对滨麦Ns基因组。SSR分析表明,DM2411可能缺失了小麦2D染色体。EST分析表明,DM2411可能含有滨麦2Ns染色体。形态学调查表明,DM2411的株高极显著降低。 |
英文摘要: |
A wheat-L.mollis alien substitution line DM2411 was identified from the F7 progenies of octoploid Tritileymus M842-16 and T.durum D4286 by using morphology,cytogenetics,genomic in situ hybridization (GISH),expressed sequence tag-sequence tagged site(EST-STS) and simple sequence repeat (SSR). Cytological studies demonstrated that DM2411 had a chromosome karyotype of 2n=42=21Ⅱ.GISH analysis indicated that DM2411 had a pair of Ns chromosomes from L.mollis. Analysis with SSR and EST-STS markers showed that the wheat 2Ds chromosomes were substituted by a pair of 2Ns chromosomes from L.mollis in DM2411. Agronomic trait evaluations showed that the plant height of DM2411 was dramatically lowered. Therefore,the substitution line DM2411 was a dwarf material,which could be exploited as an intermediate material in wheat breeding programs and genetic resources. |
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