| 叶 麟，杨 雪，张志清，申光辉，黎杉珊，吴贺君，高 玮，陈思莹.响应面法优化麦麸酯酶提取工艺[J].麦类作物学报,2017,(3):420 |
| 响应面法优化麦麸酯酶提取工艺 |
| Optimization of Extraction Process for Wheat Bran Esterase by Response Surface Methodology |
| |
| DOI：10.7606/j.issn.1009-1041.2017.03.21 |
| 中文关键词: 麦麸 植物酯酶 提取工艺 响应面优化 |
| 英文关键词:Wheat bran Plant esterase Extraction technology Response surface method |
| 基金项目:四川农业大学学科双支计划项目（03572107） |
|
| 摘要点击次数: 877 |
| 全文下载次数: 557 |
| 中文摘要: |
| 为提高小麦加工副产物麦麸的利用率，从中提取植物酯酶，并优化麦麸酯酶的提取工艺，本研究以NaNO3-磷酸缓冲液为溶剂对麦麸酯酶进行水浴静置提取，在单因素试验的基础上，利用Box-Behnken试验，进一步研究了NaNO3浓度、提取时间、提取温度3个因素的交互作用对麦麸酯酶活力的影响。最终确定麦麸酯酶的最佳提取工艺为NaNO3 浓度0.15 moL·L-1、提取温度35 ℃、提取时间60 min，此条件下单位酶活的预测值为23 338 U·mL-1，验证值为23 018.7 U·mL-1，达到预测值的98.63%。综上所述，此优化条件能够较好地从麦麸中提取植物酯酶，并保持较高的酶活性。 |
| 英文摘要: |
| In order to improve the utilization of by-product of wheat bran,plant esterase can be extracted from wheat bran. The extraction of plant esterase from wheat bran with sodium hydrogen phosphate buffer as extraction solvent was optimized. On the basis of single-factor experiments,the Box-Behnken center-united experimental design principles were used to design the experiment and the response surface analysis of three factors(concentration of NaNO3,extraction temperature and time)were determined. The optimum extraction conditions were recommended 0.15 mol·L-1 as sodium nitrate,35 ℃ as extraction temperature and 60 minutes as extraction time. Based on these conditions,the predicted value of the unit enzymatic activity was 23 338 U·mL-1,and its verified value was 23 018.7 U·mL-1,which was 98.63% agreed with the predicted value. To sum up,the optimized conditions are able to better extraction of plant esterase from wheat bran,and maintain a high enzyme activity. |
|
查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |
基本信息
