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作者	单位
沈真辉，李静烨，陈升位，莫凡，李应秋，蒲雄艳，吴海燕，黄文甫，罗平	（云南农业大学农学与生物技术学院，云南昆明 650201）

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中文摘要:

穗分枝突变体是大麦穗发育研究的重要种质。利用EMS诱变北青7号的成熟种子，再通过连续7年自交繁殖选育了9个裸大麦穗分枝突变（Ynbs)株系。在不同世代中9个Ynbs株系的穗分枝性状均可稳定遗传，多联穗明显退化，每个主穗着生2~5个分枝穗和10.53±0.53~13.23±0.22个多联穗，每个分枝穗具有1~3个分枝穗轴节，每个多联穗着生10~15个小花。为系统了解Ynbs株系的穗部特性差异，分析了不同材料多联穗数、小花数、结实粒数、结实率、穗茎轴长、穗长、穗轴节数和百粒重的表型差异和偏相关性。结果表明，多联穗数等8个穗部性状在不同材料间均有显著或极显著差异，9个Ynbs株系的多联穗数、结实率和百粒重显著低于诱变亲本，但其小花数极显著高于诱变亲本，多联穗减少、小花增多、结实率和百粒重下降是9个Ynbs株系的共有突变性状；穗分枝性状与多联穗数、结实粒数、结实率、穗长和百粒重5个穗部性状呈极显著偏相关，偏相关系数分别为-0.524、0.584、-0.646、0.418和-0.563。9个Ynbs株系具有穗分枝、多联穗退化、小穗增多等特性，是大麦穗发育等研究的不可替代种质。

英文摘要:

The mutant with branched-spike is an important germplasm for spike development study in barley. Through the EMS mutation on the mature seeds of Baiqin 7 and consecutive self-propagation of these mutated materials for seven generations,nine mutant lines with branched-spike (Ynbs lines) of naked kernel barley were developed successfully. The branched-spike of nine Ynbs lines can be inherited stably,and the degeneration of multiple-spikes on their main spike are showed in different generations. Every main spike of nine Ynbs lines has two to five branched spikes,and 10.53±0.53 to 13.23±0.22 multiple spikes. Each branched-spike contains one to three spike nodes,and each multiple-spike contains ten to fifteen florets. SPSS 19.0 was employed to analyze the phenotype difference among different materials on eight spike traits,such as multi-spike number,floret number,grain number per spike,setting percentage,length of spike stem axes,length of spike,number of spike rachis node,100-grain weight,respectively.The partial correlations between these eight traits of the spike in different materials were calculated,too.The results showed that the phenotypic value of eight spike traits show significant difference between different materials. Of these eight traits,the phenotypic value of three spike traits including number of multi-spike,setting percentage,100-grain weight are lower in nine Ynbs lines than those in mutant parent,but their floret number are all higher than that of mutant parent. All nine Ynbs lines exhibited less multi-spike,more floret,lower setting percentage and lower 100-grain weight. It showed that there are significant partial correlations between branched-spike and number of multi-spike,grain number per spike,length of spike and 100-grain weight,and correlation coefficients are -0.524,0.584,-0.646,0.418,and -0.563,respectively. Nine Ynbs lines show many characteristics including branched-spike,multi-spike degeneration and more spikelets,which can be valuable materials for spike development study in barley.

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