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作者	单位
白璐，禹飞雄，茹仙古丽·牙生，吕文娟，任毅，谢磊，耿洪伟	（1.新疆农业大学科学技术学院，新疆乌鲁木齐 830052； 2.新疆农业大学农学院，新疆农业大学生物技术重点实验室，新疆乌鲁木齐 830052； 3.新疆巴州农业局，新疆库尔勒 841000）

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中文摘要:

面粉颜色是小麦品质分级的重要指标。为了给新疆小麦面粉白度改良提供依据，本研究利用位于4BS染色体上的功能标记LOX16、LOX18和Lox-B23，对123份新疆小麦品种（系）的 TaLox-B1、 TaLox-B2和 TaLox-B3 等位变异进行分子标记检测。结果表明，在123份新疆小麦品种（系）中，具有高LOX活性等位基因 TaLox-B1a和低LOX活性等位基因 TaLox-B1b的材料分别有33份（26.8%）和90份（73.2%）；具有高LOX活性等位基因 TaLox-B2a和低LOX活性等位基因 TaLox-B2b的材料分别有122份（99.2%）和1份（0.8%）；具有高LOX活性等位基因 TaLox-B3a和低LOX活性等位基因 TaLox-B3b的材料分别有95份（77.2%）和28份（22.8%）。在不同类型冬小麦品种（系）中， TaLox-B1a的分布频率表现为引进品种（系）>自育品种（系）>地方品种外， TaLox-B3a的分布频率表现为地方品种>引进品种（系）>自育品种（系）；在不同类型春小麦中 TaLox-B1a只存在于晚期品种， TaLox-B2a和 TaLox-B3a的分布频率均表现为早期品种（100.0%）>晚期品种。所检测的新疆小麦品种(系)共有5种基因型组合，具有最高LOX活性的基因型组合 TaLox-B1a/ TaLox-B2a/ TaLox-B3a和 TaLox-B1b/ TaLox-B2a/ TaLox-B3a，具有中等LOX活性的基因型组合 TaLox-B1b/ TaLox-B2a/ TaLox-B3b和 TaLox-B1a/ TaLox-B2a/ TaLox-B3b以及具有低LOX活性的基因型组合 TaLox-B1b/ TaLox-B2b/ TaLox-B3b的分布频率分别为23.6%、53.7%、19.5%、2.4%和0.8%。在新疆小麦资源中具有高LOX活性基因型组合的材料的频率高于全国平均水平。本研究将3个位点功能标记组合起来使用，能更有效地评价检测材料的LOX活性水平。

英文摘要:

The color of the wheat flour is an important index of wheat quality traits. This study used the functional markers LOX16,LOX18 and Lox-B23,based on the development of wheat 4BS chromosome,and molecular markers were detected by TaLox-B1, TaLox-B2 and TaLox-B3 in 123 wheat varieties in Xinjiang. The results showed that,there were 33（26.8%）varieties with TaLox-B1a allele exhibited high Lox activity,90（73.2%）varieties with TaLox-B1b allele showed low Lox activity; 122（99.2%）varieties with TaLox-B2a allele exhibited high Lox activity,only 1（0.8%）varieties with TaLox-B2b allele showed low Lox activity; 95（77.2%）varieties with TaLox-B2a allele exhibited high LOX activity,28（22.8%）varieties with TaLox-B2b allele showed low Lox activity. In different types of winter wheat varieties,the frequency distribution of TaLox-B1a was in order of introduced varieties>self-cultivated varieties >local varieties. In different types of spring wheat varieties,the TaLox-B1a exists only in the late varieties,and the distribution frequency of both TaLox-B2a and TaLox-B3a was in order of early varieties (100%) >late varieties.The analysis of 3 loci genotype combinations indicated that there are 5 kinds of genetic combinations in Xinjiang wheat varieties,which were both TaLox-B1a/ TaLox-B2a/ TaLox-B3a and TaLox-B1b/ TaLox-B2a/ TaLox-B3a combinations exhibited the highest LOX activity,and were both TaLox-B1b/ TaLox-B2a/ TaLox-B3b and TaLox-B1a/ TaLox-B2a/ TaLox-B3b combinations exhibited high LOX activity,and TaLox-B1b/ TaLox-B2b/ TaLox-B3b showed the lowest LOX activity,and the distribution frequency was 23.6%,53.7%,19.5%,2.4% and 0.8%,respectively. The genotype frequency of high LOX activity in wheat cultivars in Xinjiang was higher than the national average. In this study,3 loci functional markers were combined more effectively applied to wheat quality molecular marker assisted selection.

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