| 张嘉园，亢 玲，桂安胜，李 哲，王中华，李春莲，张晓科，陈东升.小麦品系XN6426抽穗期相关基因的检测与定位[J].麦类作物学报,2016,36(11):1433 |
| 小麦品系XN6426抽穗期相关基因的检测与定位 |
| Location of the Heading Related Genes in the Wheat Line XN6426 Using the BSA Method |
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| DOI：10.7606/j.issn.1009-1041.2016.11.04 |
| 中文关键词: 小麦 抽穗期 基因定位 SSR BSA |
| 英文关键词:Wheat Heading time Location of genes SSR BSA |
| 基金项目:国家重点基础研究发展计划（973计划）项目（2104CB138102）；陕西省重点科技创新团队计划项目（2014KCT-25）；宁夏农业育种专项（2013NYYZ02）；宁夏农林科学院科技创新先导资金对外科技合作专项-小麦品质与抗逆性相关的多重PCR分子标记体系引进与利用；宁夏农林科学院科技创新先导资金基础研究项目（NKYJ-14-29）；西北农林科技大学唐仲英育种基金项目 |
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| 中文摘要: |
| 为定位小麦品系XN6426抽穗期相关基因，在可控温室（温度16~25 ℃，日光照≥16 h）和田间分别种植XN6426×京411 F2代群体、早抽穗亲本XN6426和晚抽穗亲本京411，分析F2群体抽穗期表型，得出该表型由两对基因控制。构建温室条件下F2群体的极端早抽穗和极端晚抽穗期DNA池（BSA法），采用小麦90K SNP芯片分析得出早晚抽穗期池间差异SNP位点在不同染色体上的分布频率和相应密集区域；在5A染色体上筛选出双亲和早晚抽穗期池间有多态性且分布在差异SNP位点密集区域附近的SSR标记Xbarc151和Xwmc327，这两对标记检测群体的基因型与其抽穗期表型之间极显著负相关。检测Xbarc151、Xwmc327以及亲本间有多态性的标记Xgwm186和Xwmc96在F2群体内的基因型，并结合群体相应抽穗期表型，利用复合区间作图法，在5A染色体标记Xbarc151和Xwmc327之间检测到了1个抽穗期相关QTL位点 qHD-5A-1，距离两标记的遗传距离分别为1.00 cM和11.49 cM，LOD值为3.68，贡献率为8.07%，结合前人结果初步确定该位点与 Vrn-A1位点不同，可能为已知抽穗期相关基因的未知等位变异或新基因位点。 |
| 英文摘要: |
| In order to locate the heading related genes in the wheat line XN6426,the F2 population crossed between XN6426 and Jing 411,and the parents XN6426 with early heading and Jing 411 with late heading were planted in both controlled greenhouse with high-temperature (16-25 ℃),long day light (≥16 h) and the field. The heading of the F2 population was controlled by two genes through analyzing the heading phenotype of F2 population. The early and late heading polar DNA pools (bulked segregation analysis) from the F2 population,which planted in the controlled greenhouse,were constructed. The distribution frequency of the polymorphism SNP loci on different chromosomes and the clusters to the appropriate positions were analysed by the wheat 90K SNP array. The SSR markers Xbarc151 and Xwmc327 on the 5A chromosome were detected as polymorphism between the parents and the early and late heading polar DNA pools,which were located near the chromosome clusters of the polymorphism SNP loci,and the genotype detected by the two SSR markers was significantly negative correlated with the phenotype of heading. The genotype detected by Xbarc151,Xwmc327 and the two markers Xgwm186,Xwmc96 which were polymorphism between the parents,combined with the phenotype of the population,we found one QTL qHD-5A-1 of heading between Xbarc151 and Xwmc327 with genetic distances of 1.00 cM and 11.49 cM,respectively,using the composite interval mapping method. The LOD value for this locus was 3.68. This QTL explained 8.07% of the phenotypic variation,showing that there was a heading related gene at this position. Compared with the previous researches,the site was different from the Vrn-A1 locus,and might be the new variation of the known heading related genes or the novel genes which caused the advance of heading date in the wheat line XN6426. |
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