| 郭宝健，李 赢，袁泽宸，吕 超，张新忠，许如根.大麦ARF基因家族的全基因组分析[J].麦类作物学报,2016,36(11):1426 |
| 大麦ARF基因家族的全基因组分析 |
| Genome-Wide Analysis of Auxin Response Factor (ARF) Family in Barley |
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| DOI：10.7606/j.issn.1009-1041.2016.11.03 |
| 中文关键词: 大麦 生长素响应因子 进化树 表达谱 |
| 英文关键词:Barley Auxin response factor Phylogenetic tree Expression profiling |
| 基金项目:国家自然科学基金项目（31401370；31571648）；国家大麦青稞产业技术体系建设专项（CARS-05）；江苏省农业科技自主创新基金项目(CX(14)2123)；江苏高校优势学科建设工程资助项目 |
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| 中文摘要: |
| 生长素响应因子（auxin response factor,ARF）基因家族是植物特有的转录因子家族，在调控植物生长发育过程中起到重要作用。而关于大麦ARF基因家族全基因组分析的研究尚未见报道。为进一步探讨大麦ARF基因的功能，以公布的大麦栽培品种Morex的基因组数据为基础，采用生物信息学方法鉴定了大麦ARF基因，并对其结构、染色体分布、蛋白保守结构域、系统进化树及表达谱进行了分析。结果表明，共鉴定出了17个大麦ARF基因，除4号染色体外，其余6条染色体上均有分布，片段复制事件是大麦ARF基因家族的主要扩张方式；HvARF蛋白均具有保守的B3结构域和Auxin_resp结构域，而Aux/IAA结构域仅存在于12个HvARF蛋白中，且不同蛋白所含该结构域1~2个不等；不同植物中ARF基因在功能上具有保守性；不同组织器官中HvARF基因的表达存在明显的差异。 |
| 英文摘要: |
| Auxin response factor (ARF) gene family is the specific transcription factor in plant,and plays an important role in regulation of plant organ development.However,genome-wide analysis of the ARF gene family has not been reported in barley.In this study,HvARF genes were identified based on the public genomic database of barley cultivar Morex by using bioinformatics methods.A comprehensive overview of the HvARF genes was undertaken,including gene structure,chromosome position,conserved domain of proteins,phylogenetic tree and gene expression profiling.The results showed that HvARF genes located on all chromosomes except for 4H,and segmental duplication contributed to the expansion of the ARF gene family in barley;all HvARF proteins contain a conserved B3 domain and Auxin_resp domain,and twelve HvARF proteins contain one or two Aux/IAA domains;phylogenetic classification showed that the function of ARF genes were conserved in different species;and gene expression analysis showed that the expression levels of HvARF genes were obviously different at different development stages in barley. |
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