| 刘 浩，朱维宁，张大鹏，张林生.小麦脱水素基因 WDHN1-2的克隆及其表达分析[J].麦类作物学报,2016,36(10):1291 |
| 小麦脱水素基因 WDHN1-2的克隆及其表达分析 |
| Cloning and Expression Analysis of Dehydrin Gene WDHN1-2 in Wheat |
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| DOI：10.7606/j.issn.1009-1041.2016.10.04 |
| 中文关键词: 小麦 电子克隆 脱水素 生物信息学分析 半定量RT-PCR |
| 英文关键词:Triticum aestivum L. In silico cloning Dehydrin Bioinformatics analysis Semi-quantitative RT-PCR |
| 基金项目:旱区作物逆境生物学国家重点实验室资助课题(CSBA2015007)；高等学校博士学科点专项科研基金项目(20120204110033) |
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| 中文摘要: |
| 为进一步明确小麦脱水素基因 WDHN1-2在逆境条件下的功能，以伞穗山羊草 DHN1基因为探针，通过电子克隆及RT-PCR技术获得 WDHN1-2基因后对其序列特征进行分析，同时利用基因表达综合数据库及半定量RT-PCR技术对该基因的表达模式进行解析。结果表明， WDHN1-2基因编码区(CDS)长为548 bp，编码的氨基酸具有脱水素保守序列K、Y和S片段，与山羊草脱水素EMT25371亲缘关系最近。WDHN1-2蛋白属于稳定且高度亲水蛋白，二级结构以α-螺旋和无规则卷曲为主；该蛋白在亚细胞中定位的可能性：过氧化物酶体>细胞核>线粒体基质，可能行使转录调控的功能。表达模式分析发现， WDHN1-2基因在小麦开花后22 d的胚乳中表达量最高，在ABA、PEG、NaCl及4 ℃低温胁迫下表达量均先上升后下降。 |
| 英文摘要: |
| To investigate the function of WDHN1-2 gene in wheat, WDHN1-2 was cloned from wheat in silico and analyzed by bioinformatics using DHN1 gene of Aegilops umbellulata as the probe. The CDS of WDHN1-2 gene was 548 bp,and the transcript levels of WDHN1-2 reached the highest level at 22 DAP in embryo. Semi-quantitative RT-PCR analysis indicated that transcript accumulation was first increased and then decreased under low temperature,sodium chloride (NaCl),abscisic acid (ABA) and polyethylene glycol (PEG) treatments.WDHN1-2 protein belonged to hydrophilic protein,which had typical features of DHNs that contains one Y segment,one S segment and two K segments. The secondary structure of this protein was mainly composed of alpha helix and random coil. This protein was likely to be located in the peroxisome,nucleus,and mitochondrial matrix,may play a function of transcriptional regulation. |
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