| 张爱红，崔 禹，杨 菲，邸垫平，闫 冲，苗洪芹.二穗短柄草被确定为水稻黑条矮缩病毒的新寄主[J].麦类作物学报,2016,36(8):1101 |
| 二穗短柄草被确定为水稻黑条矮缩病毒的新寄主 |
| Brachypodium distachyon,a New Host of Rice Black-Streaked Dwarf Virus |
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| DOI：10.7606/j.issn.1009-1041.2016.08.17 |
| 中文关键词: 二穗短柄草 RBSDV 寄主 |
| 英文关键词:Brachypodium distachyon Rice black-streaked dwarf virus Host |
| 基金项目:973计划项目（2014CB138400） |
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| 中文摘要: |
| 二穗短柄草（Brachypodium distachyon）是一种新兴的模式植物，在病毒-植物的互作研究中具有广阔的应用前景。水稻黑条矮缩病毒（Rice black-streaked dwarf virus,RBSDV）是一种重要的植物病毒，明确该病毒是否能够侵染二穗短柄草，是进行病毒-寄主互作研究的前提。本研究利用传毒介体灰飞虱将RBSDV人工接种于二穗短柄草Bd21，观察RBSDV是否侵染短柄草，以及侵染后的症状发展过程，同时对病毒进行了PCR检测。结果显示，RBSDV可以侵染二穗短柄草；初期症状为节间缩短，随后表现植株矮缩、心叶扭曲、缺刻等症状；PCR检测有明显的目的条带。由此确定二穗短柄草是RBSDV的新寄主，可作为该病毒与寄主互作的研究材料。这为进行RBSDV抗病基因鉴定、基因组学研究以及农作物的抗病育种奠定了基础。 |
| 英文摘要: |
| Brachypodium distachyon is a relatively ideal model plant for gramineous plants research at present.Rice black-streaked dwarf virus(RBSDV) is one of the most important virus in China, where it causes severe yield loss.Whether RBSDV infected Brachypodium distachyon or not, is the premise to establish virus-host interaction model. An inoculation method was developed to investigate the infection process and symptom development. Meanwhile, RBSDV was detected with PCR in B.distachyon.The results show that:B.distachyon could be infected by RBSDV. Typical symptoms such as stunting of plants, leaf distortion, small ears, outward-pointing notches could be observed in the plants. Furthermore, a clear single target band indicates that B.distachyon could be infected by RBSDV. According to these results, B.distachyon, a new host of RBSDV, can be used as a candidate to study virus-plant interactions. |
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