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胡雅君,栗孟飞,杨德龙,刘 媛,程洪波,常 磊,柴守玺.小麦籽粒WSC含量QTL的整合与元分析[J].麦类作物学报,2016,36(8):989
小麦籽粒WSC含量QTL的整合与元分析
Integration and Meta-analysis of Quantitative Trait Loci for Water-Soluble Carbohydrate Content in Wheat Grain
  
DOI:10.7606/j.issn.1009-1041.2016.08.03
中文关键词:  小麦  可溶性碳水化合物  一致性数量性状位点  元分析
英文关键词:Wheat  Water-soluble carbohydrates  Meta quantitative trait loci  Meta-analysis
基金项目:国家自然科学基金项目(31460348;30960195);陇原青年创新人才扶持计划项目;甘肃省农业生物技术研究与应用开发项目(GNSW-2015-18);甘肃农业大学“伏羲人才”计划项目(FXRC20130102)
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胡雅君,栗孟飞,杨德龙,刘 媛,程洪波,常 磊,柴守玺 (1.甘肃省干旱生境作物学重点实验室/甘肃农业大学生命科学技术学院甘肃兰州 730070 2.甘肃农业大学农学院甘肃兰州 730070) 
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中文摘要:
      在干旱胁迫条件下,小麦营养器官暂贮性可溶性碳水化合物(Water-soluble carbohydrates,WSC)是小麦籽粒灌浆所需的重要碳源。为发掘控制小麦籽粒WSC含量的真实主效数量性状位点(Quantitative trait loci,QTL),利用生物信息学方法,借助小麦高密度分子标记遗传图谱,对来自不同遗传作图群体的控制小麦籽粒WSC含量的168个QTL位点进行图谱映射和元分析。结果发现,142个QTL定位区间与参考图谱有共有标记,其中92个QTL对籽粒WSC含量的表型变异具有增效效应,50个QTL具有减效效应。建立控制小麦籽粒WSC含量的QTL一致性图谱,获得16个“一致性”QTL (Meta quantitative trait loci,MQTL)位点及其连锁标记,MQTL置信区间最小达到0.77 cM。
英文摘要:
      Under drought stress conditions,water-soluble carbohydrates (WSC) of vegetative organs in wheat are an important source of carbon required for wheat grain filling. To explore true and major quantitative trait loci (QTL) for WSC content in wheat grain,a total of 168 QTL controlling grain WSC content from different genetic mapping populations in wheat were employed to establish consensus map and to perform meta-analysis by the method of bioinformatics,depending on a high-density genetic map as a reference map. There were 142 QTL overlapped the same marker intervals with the reference map.Of them,ninty-two QTL had positive effects on phenotypic variations of grain WSC content in wheat,whereas 50 QTL showed negative effects. A consensus genetic map for QTL controlling grain WSC content was established. Furthermore,sixteen meta QTL (MQTL) and their tightly linked markers were confirmed,with the minimum confidence interval of MQTL reaching to 0.77 cM.The findings might provide theoretical basis for fine mapping and map cloning of QTL controlling grain WSC content in wheat.
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