| In order to clarify the expression of genes related to antioxidant enzymes under cryogenic conditions,and reveal the physiological mechanism of cold resistance in winter wheat,Dongnongdongmai 1 and Chinese Spring,with distinguishing cold resistance were used as materials to identify the expression differences of TSOD-1, TSOD-2, TSOD-3 related to superoxide dismutase(SOD) and HAPX-1, HAPX-2, HAPX-3 related to ascorbate peroxidase(APX) under different low temperature.Results showed that,under specific low temperature treatment,the expression level of TSOD-1, TSOD-2, TSOD-3, HAPX-2 and HAPX-3 genes in Dongnongdongmai 1 were significantly higher than that in Chinese Spring,while the expression level of HAPX-1 gene in Dongnongdongmai 1 was almost lower than that in Chinese Spring.Among them,the relative expression level of TSOD-3 gene in Dongnongdongmai 1 with respect to that in Chinese Spring reached the maximum at the beginning of the cold processing (cold acclimation 30 d,-10 ℃ for 2 h),with a ratio of 8.63 and 11.78,respectively.However,the relative expression level of HAPX-2 and HAPX-2 gene in Dongnongdongmai 1 relative to that in Chinese Spring reached the maximum at the frozen period (-12 ℃ for 2 h),with a ratio of 3.12 and 21.22,respectively.It is conclued that TSOD-3, HAPX-2,and HAPX-3 genes are important genes involved in cold response.Of them, TSOD-3 gene plays a major role in the early stage of cold stress,and HAPX-2 and HAPX-3 continue to play a major role in refrigeration. |
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