| BNS is a new type of thermo-sensitive wheat male-sterile line,and has excellent sterility and convertibility by itself. It is of important value for the utilization of hybrid wheat and the research of genetic resources. In order to locate the restorer gene of BNS,wheat Chinese Spring(CS),a high restorer line for BNS,was selected as parent material to reform an F2 population by crossing over BNS. In the detection,two bulked segregant analysis(BSA) pools of phenotypes,the self-seedsetting rate and pollen fertility rate were established. First,the linkage groups related to restorer gene(s) were detected by Chinese Spring nulli-tetrasomes. Then,SSR molecular markers on these linkage groups were used to screen two BSA pools,and the linked markers obtained in screening were used to screen F2 population to detect QTL loci of restorer gene(s). The results showed that when CS nulli-tetrasomes was crossed over BNS,there were four recombinations in F1 to be pulled into low self-seedsetting rate,meaning that the four linkage groups on chromosomes 1B,2B,1A and 7B were relative to the recovery of BNS. When 222 pairs of SSR primers from 8 chromosomes were used to screen two phenotypes BSA pools,it was found that there were eight SSR markers on three linkage groups on chromosomes 1B,2B and 1A,which are linked with the restoration of BNS. These eight linkage markers then were used for screening 210 individual plants of F2 population,and five QTL loci were found. Of them,one QTL was relative to self seedsetting rate only,and two major QTLs were relative to two phenotypes,and two minor QTLs were relative to fertile pollen rate only,which were located on chromosomes 1A,1B and 2B,respectively. These results laid a value foundation for MAS(marker-assisted selection) and fine mapping of BNS restorer genes. |
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