| For a better understanding of common wheat quality breeding,three spring wheat varieties/lines,Ningchun 4,Ningchun 44,and J058,and their introgression lines created by pollen-tube pathway using genomic DNA of soybean varieties Tiefeng 31,Chengdou 6,Fendou 78 and Tie 9808,respectively,were used.Quality traits,such as protein content,wet gluten content and sedimentation were analyzed and plant height,spike length,thousand-kernel weight,grains per spike and other agronomic traits were also investigated after harvest at the mature period during 2012-2014.Besides,RAPD marker analysis was conducted.The results showed that related agronomic traits and quality traits of introgression lines had changed in comparison with parents.The economic coefficient and the thousand-kernel weight of introgression line 019 were significantly higher than those of the parent Ningchun 4 with an increase rate of 14.33% and 14.84%,respectively.In 2012,quality traits of introgression line 013 were reached highest level in all tested lines.Multiple comparison results revealed that only the protein content of introgression line 013 reached significant level,with the increase rate of 13.33%,compared to the parent Ningchun 4.Eighteen random primers were employed to amplify genomic DNA of the donor soybeans and the receptor wheat materials as well as their introgression lines.Of them,three RAPD markers gave clear bands to separate the soybeans from wheat evidently.Polymorphic bands were detected between wheat parents and soybeans as well as part of introgression lines which had donor bands,such as introgression lines 013,015,019 and 020.The introgression line 013 which had remarkable agronomic traits and improved protein content was finally selected as a new material for future study. |
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