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作者	单位
刘志萍，李琲琲，薛海楠，张凤英，包海柱，王聪，徐寿军	（1.内蒙古农牧业科学研究院作物所，内蒙古呼和浩特 010000； 2.内蒙古民族大学农学院，内蒙古通辽 028043）

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中文摘要:

为探讨NaCl胁迫对大麦籽粒抗坏血酸(AsA)-谷胱甘肽（GSH）循环的影响，以耐盐品系11pj-173和盐敏感品系11pj-033为试验材料，研究了不同浓度NaCl胁迫下，大麦籽粒灌浆期AsA-GSH循环的变化规律。结果显示，随着NaCl胁迫天数的增加，2个大麦品系的H₂O₂含量均逐渐增加，但11pj-173较同期对照的增幅均小于11pj-033；抗坏血酸过氧化物酶(APX)和谷胱甘肽还原酶(GR)活性、AsA、氧化型抗坏血酸(DHA)、GSH、氧化型谷胱甘肽(GSSG)含量以及AsA/DHA比值均呈先升后降趋势。11pj-173的GSH/GSSG比值呈先升后降的趋势，而11pj-033呈波状变化，即先升后降又略增。与同期对照比，11pj-173的APX和GR活性、AsA和GSH含量及AsA/DHA和GSH/GSSG的增幅均高于11pj-033，降幅均小于11pj-033；DHA和GSSG含量则与之相反，表明11pj-173籽粒在胁迫期间能够保持较高的AsA-GSH循环效率,可有效地抑制 H₂O₂的积累。

英文摘要:

The changes of antioxidant enzyme activity and ascorbate glutathione cycle were studied in barley seeds with salt tolerant cultivar(11pj-173) and salt sensitive cultivar(11pj-033) as experimental materials.The results showed that the H₂O₂ content increased gradually with the increase of NaCl stressed days.Compared with the control at same period,the H₂O₂ content increase magnitude of 11pj-173 was less than 11pj-033’s; The activity of GR and APX,the content of AsA,DHA,GSH and GSSG,and the ratios of AsA/DHA increased at first and then decreased;The GSH/GSSG ratios of 11pj-173 increased at first and then decreased,and the GSH/GSSG ratios of 11pj-033 showed a wavy change. Compared with the control at same period,the increase magnitude of APX and GR activity,AsA and GSH content,AsA/DHA and GSH/GSSG ratios of 11pj-173 was more than 11pj-033’s,and its reduced amplitude was less than 11pj-033’s; The content of DHA and GSSG was the opposite. These results indicate that 11pj-173 could maintain efficient metabolism of AsA-GSH cycle during the strss period,and further maintain lower H₂O₂ accumilation.

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