| 胡文祥，杨帼一， 刘 童， 赵惠贤， 刘香利.二穗短柄草成熟胚再生体系建立及农杆菌介导转化研究[J].麦类作物学报,2016,36(5):589 |
| 二穗短柄草成熟胚再生体系建立及农杆菌介导转化研究 |
| Establishment of Brachypodium distachyon Regeneration System from Mature Embryos and the Study of Agrobacterium-mediated Transformation |
| |
| DOI：10.7606/j.issn.1009-1041.2016.05.08 |
| 中文关键词: 二穗短柄草 成熟胚 愈伤组织 农杆菌 |
| 英文关键词:Brachypodium distachyon Mature embryo Callus Agrobacterium |
| 基金项目:国家自然科学基金项目（31101205）；国家生命科学与技术人才培养基地大学生创新性实验计划项目（1310712082） |
|
| 摘要点击次数: 1261 |
| 全文下载次数: 666 |
| 中文摘要: |
| 为建立二穗短柄草组织培养及遗传转化体系，以二穗短柄草BD21-3成熟胚为外植体，对成熟胚愈伤诱导、分化以及农杆菌侵染条件进行了研究。结果表明，在含有2.5 mg·L-1 2，4-D的培养基上，愈伤组织出愈率最高为93.83%；在含有0.2 mg·L-1 KT的分化培养基上，分化率最高为38.18%；对二穗短柄草胚性愈伤组织农杆菌转化和GUS染色结果表明，侵染的过程中农杆菌菌液浓度OD600为0.6、侵染时间为 5 min 时转化率最高。 |
| 英文摘要: |
| To establish the regeneration and genetic transformation system of Brachypodium distachyon,the mature embryos of Brachypodium distachyon BD21-3 were used as explant,and the factors affecting the callus induction and the Agrobacterium infection were studied in this research. The results showed that the frequency of callus induction was 93.83% on the basic media with 2.5 mg·L-1 2,4-D. The maximum regeneration frequency (38.18%) was observed on the differential media containing 0.2 mg·L-1 KT. The GUS staining showed that the highest GUS activity was obtained when the concentration of Agrobacterium was OD600=0.6 and infection incubation duration was 5 min. |
|
查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |
基本信息
