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作者	单位
李信，杨晓菲，马兵，陈志刚，王长有，陈春环，田增荣，吉万全	（1.西北农林科技大学农学院/旱区作物逆境生物学国家重点实验室,陕西杨凌 712100 2.陕西省种子管理站,陕西西安 710021；3.陕西省兴平市种子管理站，陕西兴平 713100）

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中文摘要:

为了有效挖掘滨麦优异的基因资源，利用形态学、细胞学、荧光原位杂交 (FISH)、基因组原位杂交(GISH) 和分子标记等技术，对小麦-滨麦衍生后代M13063-3-3进行了鉴定。结果表明，M13063-3-3的染色体构型为2n＝44＝22Ⅱ。以滨麦基因组DNA为探针的原位杂交结果显示，二条染色体有杂交信号，且能配对，表明两条外源染色体是滨麦的一对同源染色体。SSR、EST和PLUG标记分析表明，M13063-3-3附加的滨麦染色体归属于第6部分同源群染色体。A-PAGE电泳分析表明，M13063-3-3在α区具有滨麦染色体特征带，进一步验证了附加的滨麦染色体与小麦第6部分同源群染色体有部分同源关系。田间条锈病调查结果表明，M13063-3-3对条锈菌生理小种条中31号、条中32号和条中33号混合菌种表现高抗。因此，M13063-3-3是一个附加了一对滨麦第6部分同源群染色体的抗条锈病的普通小麦材料，为小麦抗病育种提供了新的种质资源。

英文摘要:

Exploiting and utilizing excellent gene(s) from wild species has become an essential strategy for wheat improvement.The derivative line M13063-3-3,which was developed from the progeny of common wheat (Triticum aestivum L.) cv.7182 and Leymus mollis,was characterized based on cytological observation,fluorescence in situ hybridization (FISH),genomic in situ hybridization (GISH),simple sequence repeat (SSR),expressed sequence tag (EST),PCR-based landmark unique gene (PLUG) markers and morphological analysis.M13063-3-3 contained 22 bivalents during meiosis,while GISH results detected two alien chromosomes.Three SSR markers,three EST markers and two PLUG markers,which were located on the homoeologous group 6 chromosomes of wheat,amplified polymorphic bands in the derivative line M13063-3-3,which were unique to L.mollis.A-PAGE indicated that α region of M13063-3-3 had the specific band of L.mollis.The results further showed that the additive chromosomes belong to the homoeologous group 6 chromosomes of L.mollis.After inoculation using the mixed races of stripe rust (CYR31,CYR32 and CYR33) at the adult stage,M13063-3-3 exhibited high resistance to stripe rust,which was possibly derived from L.mollis.The high levels of disease resistance make M13063-3-3 a promising germplasm for wheat breeding.

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