| 贺晓岚，王建伟，赵继新，李文旭，武 军，陈新宏.滨麦蔗糖:果聚糖6-果糖基转移酶（6-SFT）基因全长cDNA的克隆与生物信息学分析[J].麦类作物学报,2016,36(5):549 |
| 滨麦蔗糖:果聚糖6-果糖基转移酶（6-SFT）基因全长cDNA的克隆与生物信息学分析 |
| Cloning and Bioinformatics Analysis of cDNA of Sucrose:Fructan-6-Fructosyltransferase (6-SFT) Gene from Leymus mollis |
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| DOI：10.7606/j.issn.1009-1041.2016.05.02 |
| 中文关键词: 滨麦 6-SFT基因 克隆 序列分析 |
| 英文关键词:Leymus mollis 6-SFT gene Clone Sequence analysis |
| 基金项目:国家自然科学基金项目（31571650）；西北农林科技大学唐仲英育种基金项目 |
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| 中文摘要: |
| 为了挖掘和利用滨麦（Leymus mollis,2n=4x=28,JJNN）的优良基因，以拓宽小麦非生物胁迫抗性基因资源，以滨麦为材料，利用RT-PCR结合RACE技术从滨麦叶片中克隆到 6-SFT基因cDNA的全长序列，并对其进行生物信息学分析。结果表明，其cDNA全长为2 086 bp，开放阅读框为1 866 bp（命名为 Lm-6-SFT），编码621个氨基酸；其推导的蛋白分子量为69.1 kDa，理论等电点（pI）为5.18，属于酸性蛋白。保守结构域分析表明，该基因推导的氨基酸序列含有SDPDG、RDP和EC结构域。多序列比对及进化树分析表明，滨麦6-SFT与冰草6-SFT在氨基酸水平具有高度的序列相似性。 |
| 英文摘要: |
| In order to explore the abiotic stress resistance gene of Leymus mollis (2n=4x=28,JJNN) and broaden the abiotic stress resistance gene resources in wheat,the reverse transcription PCR (RT-PCR) and rapid-amplification of cDNA ends (RACE) techniques were used to clone a full-length cDNA sequence of Lm-6-SFT gene from L.mollis leaves,and the bioinformatics analysis was conducted. The results showed that the full-length cDNA sequence,designated as Lm-6-SFT,with 2 086 bp in length,contains an open reading frame (ORF) with 1 866 bp. Lm-6-SFT was predicted to encode a 621 amino acids protein with molecular weight of 69.1 kDa and isoelectric point of 5.18,which belongs to the acidic protein. The deduced amino acids contain SDPDG,RDP,and EC conservative domains. Multiple sequence alignment and phylogenetic analysis showed that the Lm-6-SFT protein shared high similarity with 6-SFT proteins from Agropyron cristatum. |
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