| 徐 姣，刘 佳，代君丽，李洪连.中国黄淮麦区菲利普孢囊线虫淮阳和博爱致病型群体特异性SCAR标记的建立[J].麦类作物学报,2016,36(4):523 |
| 中国黄淮麦区菲利普孢囊线虫淮阳和博爱致病型群体特异性SCAR标记的建立 |
| Development of SCAR Marker for Detecting Heterodera filipjevi of Huaiyang and Boai Pathotype Populations from Wheat-growing Regions of the Huang-Huai-Hai Plain in China |
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| DOI：10.7606/j.issn.1009-1041.2016.04.19 |
| 中文关键词: 小麦 菲利普孢囊线虫 致病型 分子检测 |
| 英文关键词:Wheat Heterodera filipjevi Pathotype Molecular detection |
| 基金项目:国家自然科学基金项目(31101419)；国家公益性（农业）科研专项（200903040-4） |
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| 中文摘要: |
| 菲利普孢囊线虫（Heterodera filipjevi）是在我国黄淮麦区新发现的小麦病原线虫，其致病力更强，危害更严重，对小麦产量和质量造成潜在威胁。病原致病型鉴定对抗病品种的筛选和培育十分重要。为了建立一套简便、快速、准确的SCAR标记检测体系，以黄淮麦区5个重要禾谷孢囊线虫致病型共9个群体为材料，首先对其进行RAPD分析，然后在RAPD分析的基础上对其进行SCAR分析。结果表明，通过筛选97条随机引物，获得2个菲利普孢囊线虫淮阳和博爱致病型群体特异的RAPD标记，其中，引物S232扩增出的多态性条带大小为550 bp，引物S278扩增出的多态性条带大小为1 700 bp。其后将引物S232扩增的条带进行回收、克隆及测序，根据测序结果设计的特异性引物F232-8-1/R232-8-1仅在菲利普孢囊线虫淮阳和博爱致病型群体中扩增出大小为517 bp的特异性条带，而在小麦孢囊线虫其他致病型群体中没有扩增出该条带，说明菲利普孢囊线虫淮阳和博爱致病型群体SCAR标记成功建立，并将其命名为SC-S232。 |
| 英文摘要: |
| Heterodera filipjevi was discovered in the Huang-Huai-Hai Plain in China and is a new pathogenic nematode of wheat. It seriously threatens wheat production due to the capability of high pathogenicity. Identification of pathogenic pathotype is quite important for screening and breeding of disease-resistant wheat cultivars.In order to establish a kind of rapid,convenient,and accurate method based on SCAR markers,random amplified polymorphism DNA(RAPD) techniques was used to analyze five pathotypes,including nine cereal cyst nematode(CCN) populations. A total of 97 random primers were screened and 2 of them,S232 and S278,produced distinct bands that were specific for Huaiyang and Boai populations of H.filipjevi based on RAPD method. The two specific bands were 550 base pair(bp),and 1 700 bp in size,respectively. Subsequently,a SCAR marker SC-S232 was developed according to the RAPD marker S232. The results showed that SC-S232 marker amplifies a positive band with a size of 517 bp from the Huangyang and Boai pathotype populations of H.filipjevi,but none product from other pathotype populations of CCN in the Huang-Huai-Hai plain. This SCAR marker SC-S232 can be used to detect the Huangyang and Boai pathotype populations in this region. |
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