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周 强,袁中伟,欧俊梅,任 勇,杜小英,陶 军,李生荣,刘登才.四川小麦主要矮秆基因的分子鉴定[J].麦类作物学报,2015,35(12):1624
四川小麦主要矮秆基因的分子鉴定
Molecular Identification of the Main Dwarfing Genes in Wheat Varieties in Sichuan
  
DOI:10.7606/j.issn.1009-1041.2015.12.03
中文关键词:  小麦  四川  矮秆基因  分子标记
英文关键词:Wheat  Sichuan  Dwarfing Gene  Molecular marker
基金项目:国家现代农业产业技术体系建设专项(CARS-3-2-40);四川省“十二五”育种攻关项目(2011YZGG-3);国家现代农业产业技术体系四川省麦类创新团队项目
作者单位
周 强,袁中伟,欧俊梅,任 勇,杜小英,陶 军,李生荣,刘登才 (1.四川农业大学小麦研究所四川成都 611130 2.绵阳市农业科学研究院,国家小麦改良中心绵阳分中心四川绵阳 621023 3.农业部小麦水稻等作物遗传育种重点实验室四川绵阳 621023) 
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中文摘要:
      为系统研究四川小麦品种主要矮秆基因的组成,应用 Rht-B1bRht-D1b基因的STS标记及 Rht8基因的连锁SSR标记,对262个小麦品种(系)进行了检测。结果表明,四川小麦品种(系)具有 Rht8基因连锁标记的频率为54.6%, Rht-D1b基因的频率为37.4%, Rht-B1b基因的频率为15.3%。与前人的研究比较发现,四川小麦 Rht8连锁标记的频率高于全国平均值,而 Rht-B1bRht-D1b的频率都低于全国平均值。这3种矮秆基因一共构成了8种组合类型。为分析不同基因组合对株高的影响,对连续种植3年的125份材料进行了测定。这些材料中,不含3种基因的类型占12.8%,仅含有 Rht8的类型最多(33.6%),其次为仅含有 Rht-D1b基因的类型(20%);同时含有 Rht8基因和 Rht-D1b基因的类型占17.6%;含有 Rht-B1b基因的组合类型所占比例较少(都低于10%)。矮秆基因的降秆强度表现为: Rht-D1b> Rht-B1b> Rht8。同时具有3种矮秆基因的材料,其降秆强度最大,其次为含有2种矮秆基因的组合类型。
英文摘要:
      In order to investigate the distribution of the three main dwarfing genes, Rht-B1b, Rht-D1b and Rht8 in Sichuan,262 wheat varieties were detected using STS markers for gene Rht-B1b and Rht-D1b and SSR marker linked to Rht8. The results showed that the frequency was 54.6% for Rht8,37.4% for Rht-D1b,and 15.3% for Rht-B1b. This distribution frequency was similar to the previously studies reported in Southwestern winter wheat region.Compared to the national average level,the frequency of Rht8 in Sichuan was higher,but the frequency for Rht-B1b and Rht-D1b was lower.The three Rht genes constituted eight combinations. In order to investigate the effects of Rht genes on plant height,125 varieties were planted in successive for three years. Out of the analyzed 125 varieties,12.8% had none Rht gene,33.6% had Rht8 only,and 20% had Rht-D1b only, and 17.6% had both Rht8 and Rht-D1b,and those combinations with Rht-B1b gene accounted for less than 10%. The range of plant height reduction ability for Rht gene was:Rht-D1b> Rht-B1b> Rht8.As for the combinations,the varieties carrying all three genes exhibited a highest ability to reduce plant height,while those with single gene showed a lowest ability.
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