| 王洋洋，邱 玲，李 萌，汤述尧，符书兰，唐宗祥.小麦品种绵麦37和绵麦367抗白粉病基因的FISH分析及分子检测[J].麦类作物学报,2015,35(11):1502 |
| 小麦品种绵麦37和绵麦367抗白粉病基因的FISH分析及分子检测 |
| Molecular Detection and FISH Analysis of Powdery Mildew Resistance Gene in Wheat Cultivars Mianmai 37 and Mianmai 367 |
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| DOI：10.7606/j.issn.1009-1041.2015.11.06 |
| 中文关键词: 小麦 白粉病 Pm21基因 FISH |
| 英文关键词:Wheat Powdery mildew Pm21 gene FISH |
| 基金项目:国家自然科学基金项目（31470409） |
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| 摘要点击次数: 1968 |
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| 中文摘要: |
| 为了进一步研究和利用小麦品种绵麦37和绵麦367所携带的白粉病抗性基因，首先以Oligo-pSc119.2-1（可检测小麦染色体结构变异）、Oligo-pTa535-1（可检测小麦染色体结构变异）和pDb12H（可检测簇毛麦染色体）为探针，对这两个小麦品种进行了荧光原位杂交（FISH）分析，然后利用与 Pm21基因连锁的分子标记NAU/xibao15对这两个小麦品种进行分子检测。结果表明，绵麦37和绵麦367都含有1对 6VS/6AL易位染色体，且均携带 Pm21基因。 |
| 英文摘要: |
| For further utilization of powdery mildew resistant gene(s) in wheat cultivars Mianmai 37 and Mianmai 367, fluorescence in situ hybridization (FISH) by using probes Oligo-pSc119.2-1 (to detect structural variations of wheat chromosomes),Oligo-pTa535-1 (to detect structural variations of wheat chromosomes) and pDb12H (to detect chromosomes of Dasypyrum breviaristatum) was first used to analyze Mianmai 37 and Mianmai 367. Subsequently,molecular marker NAU/xibao15,which is linked toPm21 gene,was also used to analyze the two wheat cultivars. The results indicated that the two wheat cultivars contained a pair of 6VS/6AL translocation chromosomes andPm21 gene. |
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