| 安旭尧，刘 聪，朱传杰，孙道杰，冯 毅，张玲丽.黄化小麦近等基因系遗传背景、光合和农艺性状分析[J].麦类作物学报,2015,35(11):1476 |
| 黄化小麦近等基因系遗传背景、光合和农艺性状分析 |
| Evaluation and Analysis of the Main Photosynthetic Properties and Agronomic Characteristics of Three Xantha Wheat NILs |
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| DOI：10.7606/j.issn.1009-1041.2015.11.02 |
| 中文关键词: 小麦 黄化突变体 近等基因系 遗传背景 光合特性 农艺性状 |
| 英文关键词:Wheat Xantha mutant NIL Genetic background Photosynthetic properties Agronomic characteristics |
| 基金项目:陕西省自然科学基金项目（2013JM3007）；西北农林科技大学基本科研业务费专项（Z109021563）；“十二五”科技部国家科技计划项目（2013BAD01B02） |
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| 中文摘要: |
| 为了解新创制的黄化小麦近等基因系（黄化系1-20YY、黄绿系1-20YG和绿色系1-20GG）的遗传背景、光合及农艺性状特点，利用分布于小麦基因组上的168个SSR分子标记和种子醇溶蛋白A-PAGE技术对其进行遗传鉴定，同时对旗叶光合性能及主要农艺性状进行分析。结果表明，返青后3个近等基因系间叶色差异显著，但其遗传背景高度一致；选用的168个SSR引物中仅在一个位于2BS上的cfd238位点检测到多态性，其余引物检测结果均为单态；3个近等基因系间在种子醇溶蛋白组成上完全相同。近等基因系间旗叶光合色素含量差异显著，其中黄化系1-20YY为叶绿素严重缺乏突变体，光合性能最差，不能完成整个生育期而提早死亡；黄绿系1-20YG也属叶绿素缺乏突变体，虽然其光合性能受影响较小，生长发育正常，但单株生产能力较绿色系1-20GG显著下降。 |
| 英文摘要: |
| The three xantha wheat near-isogenic lines (NILs)( a yellow leaf mutant line 1-20YY,a yellow-green leaf mutant line 1-20YG and a normal green leaf line 1-20GG) obtained by 13 generation self-crossing were studied. Examination of the NILs with 168 SSR molecular markers and seed gliadin’s A-PAGE revealed that the three NILs had almost the same genetic background. Among the SSR markers analyzed,One (cfd238) of them,which located on wheat chromosome 2BS,was polymorphic,and others were monomorphic. Homogeneity in gliadin banding patterns was observed of the NILs.The pigment contents of three NILs were significantly different. Difference of the photosynthetic pigment content among the NILs was significant.The yellow leaf mutant line 1-20YY was seriously chlorophyll-less mutant. Photosynthetic performance of the line 1-20YY was the worst,which seriously affected the normal growth and development during.The line 1-20YG also belonged to the chlorophyll-reduced mutant,but little photosynthetic performance of 1-20YG was affected.The growth and development of the line 1-20YG was normal,and the plant productivity per plant of it was reduced prominently. |
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