| 寇 程，李学军，高 欣，吕 千，杜登峰，程 显，李 扬，李立群.小麦粒重基因TaGW2-6A等位变异的高通量分子检测[J].麦类作物学报,2015,35(11):1467 |
| 小麦粒重基因TaGW2-6A等位变异的高通量分子检测 |
| High-throughput Molecular Detection of Allelic Variation in TaGW2-6A Gene of Wheat (Triticum aestivum L.) |
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| DOI：10.7606/j.issn.1009-1041.2015.11.01 |
| 中文关键词: 小麦 TaGW2-6A基因 等位变异 高分辨率熔解曲线(HRM) |
| 英文关键词:Wheat TaGW2 gene Allelic variation High resolution melting curve(HRM) |
| 基金项目:农业部高产转基因小麦新品种培育科技重大专项(2013ZX08002-003)；陕西省科技统筹创新工程计划项目(2014KTZB02-01-01) |
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| 中文摘要: |
| 为了开发出一种高通量的TaGW2-6A基因等位变异分子检测技术，利用高分辨率熔解曲线分析技术(High resolution melting curve analysis,HRM)检测了316份小麦材料的TaGW2-6A基因编码区T碱基插入等位变异以及启动子区Hap-6A-A(-593A和-739G)和Hap-6A-G(-593G和-739A)等位变异，同时利用桑格测序和Hap-6A-P1/P2分子标记验证HRM的分析结果。结果表明，HRM技术能够准确、高效的检测TaGW2-6A基因的等位变异。在316份材料中，检测到61份T碱基插入等位变异材料（命名为TT基因型）和255份无T碱基插入的等位变异材料（命名为tt基因型）；其中，189份为Hap-6A-A单倍型材料，127份为Hap-6A-G单倍型材料。 |
| 英文摘要: |
| TaGW2 on chromosome 6A is a key gene governing kernel size of wheat (Triticum aestivum L.). A single T-base insertion has been found in the eighth exon of TaGW2 (977 bp) and two SNPs (Hap-6A-A and Hap-6A-G) in the promoter region. In this study,a high-throughput technology using high resolution melting curve (HRM) analysis was established and used to detect the allelic variations in TaGW2-6A allelic gene among 316 wheat materials.Results of HRM analysis were verified by Sanger sequencing and molecular marker for Hap-6A-P1/P2. HRM can detect the allelic variation in TaGW2-6A gene accurately and efficiently. There were totally 61 wheat materials detected as containing T-base insertion (TT genotype) and 255 wheat materials detected as lacking of T-base insertion (tt genotype),meanwhile,189 and 127 wheat materials were detected as Hap-6A-A haplotype and Hap-6A-G haplotype,respectively. |
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