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作者	单位
韩颜超，王长有，陈春环，田增荣，吉万全	（旱区作物逆境生物学国家重点实验室/西北农林科技大学农学院，陕西杨凌 712100）

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中文摘要:

为给后续研究和利用普通小麦-华山新麦草衍生后代H1-1-3-1-9-8提供依据，用形态学、细胞学及分子生物学方法对该材料进行分析研究。结果表明，H1-1-3-1-9-8在株高、穗长、穗型等性状上与华山新麦草无显著差异，千粒重平均44.2 g，较亲本显著增加；H1-1-3-1-9-8的染色体构型为2n=44=22Ⅱ；经原位杂交鉴定，H1-1-3-1-9-8存在两条华山新麦草外源信号，且能够配对；选取普通小麦7个部分同源群上的64对EST-STS引物对H1-1-3-1-9-8进行分析，第一部分同源群上的BE443796、BE446010、BE497584、BF202643、BG262410、BG607965和BM140362七对引物扩增出了华山新麦草的特异条带；经种子贮藏蛋白变性聚丙烯酰胺凝胶电泳分析，H1-1-3-1-9-8具有华山新麦草特有的高分子量谷蛋白亚基。以上结果说明，H1-1-3-1-9-8是普通小麦-华山新麦草1Ns二体异附加系。

英文摘要:

To provide the basis for further research and utilization of derivative of wheat-Psathyrostachys huashanica Keng H1-1-3-1-9-8, study has been conducted with morphologic, cytological, and molecular biological methods. Results indicated that stem length, spike length and spike sharp of H1-1-3-1-9-8 were similar to P.huashanica Keng, but thousand kernel weight was 44.2 g, which was superior to the parent. Cytology analysis showed that the chromosome configuration was 2n=44=22Ⅱ.Genomic in situ hybridization (GISH) analysis showed that the materials had a pair of Ns chromosomes with yellow-green signals. 64 pairs of STS markers distributed in seven homoeologous groups of wheat were selected to identify the Ns chromosome. Seven markers including BE443796, BE446010, BE497584, BF202643, BG262410, BG607965 and BM140362, which were mapped on homoeologous group 1 chromosomes, amplified specific bands for P.huashanica Keng. Unique bands of high-molecular-weight glutenin subunit specific from P.huashanica were detected by SDS-PAGE. Thus, it can be concluded that H1-1-3-1-9-8 is a wheat-P.huashanica Keng 1Ns disomic addition line.

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