| 孙德祥，秦海霞，丁会纳，马冬云，李耀光，王晨阳，郭天财.硅肥对干旱胁迫下冬小麦旗叶抗坏血酸-谷胱甘肽循环系统及产量的影响[J].麦类作物学报,2015,35(5):693 |
| 硅肥对干旱胁迫下冬小麦旗叶抗坏血酸-谷胱甘肽循环系统及产量的影响 |
| Effect of Silicon Fertilizer on Ascorbic Acid and Glutathione Circulatory System in Flag Leaf and Yield of Winter Wheat under Drought Stress |
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| DOI：10.7606/j.issn.1009-1041.2015.05.18 |
| 中文关键词: 冬小麦 硅肥 干旱胁迫 抗坏血酸-谷胱甘肽循环 产量 |
| 英文关键词:Winter wheat Exogenous silicon fertilizer Drought stress Ascorbic acid and glutathione circulatory system Yield |
| 基金项目:农业部公益性行业专项(201203031); 国家十二五科技支撑计划项目(2012BAD04B07); 现代农业产业体系项目(CARS-03) |
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| 中文摘要: |
| 为从抗坏血酸-谷胱甘肽循环的抗氧化角度提示硅元素的抗旱机理，以冬小麦品种豫麦49-198为材料，研究了硅肥对不同干旱胁迫（对照、轻度胁迫、重度胁迫）下小麦旗叶抗坏血酸-谷胱甘肽循环系统中关键基因表达、抗氧化物质含量及产量的影响。结果发现， 在轻度和重度胁迫持续10 d时，施用硅肥与不施用硅肥比较，小麦旗叶抗坏血酸分别提高了16.57%和8.39%，小麦旗叶中抗坏血酸-谷胱甘肽循环系统关键酶基因TaAPX、TaGR、TaGS相对表达量分别提高了60.23%、73.61%、11.35%和87.97%、25.70%、114.64%；在轻度胁迫和重度胁迫持续20 d时，施用硅肥与不施用硅肥比较，小麦旗叶抗坏血酸含量分别提高了18.03%和6.81%，小麦旗叶中抗坏血酸-谷胱甘肽循环系统关键酶基因TaAPX、TaGR、TaGS相对表达量分别提高了124.54%、44.42%、56.80%和118.19%、41.42%、269.21%；小麦籽粒产量分别提高了29.23%和33.31%。以上结果说明，施用硅肥可以增强小麦旗叶抗坏血酸-谷胱甘肽循环系统功能，提高小麦植株的抗旱性，增加籽粒产量。 |
| 英文摘要: |
| Experiments were carried out to study the effects of exogenous silicon fertilizer on the expression of the key genes,the antioxidant contents in ascorbic acid and glutathione circulatory system of the flag leaf and the yield of winter wheat cultivar Yumai 49-198 under different drought stress. The results showed that compared to the control of none silicon applied,applying silicon fetilizer increased the content of ascorbic acid by 16.57% under light drought stress for 10 days and increased by 18.03% for 20 days，increased by 8.39% under serious drought stress for 10 days and increased by 6.81% for 20 days；The expression of TaAPX,TaGR,TaGS were enhanced by 60.23%,73.61%,11.35% under light drought stress for 10 days and enhanced by 124.54%,44.42%,56.80% for 20 days,enhanced by 87.97%,25.70%,114.64% under serious drought stress for 10 days and enhanced by 118.19%,41.42% ,269.21% for 20 days ; and the yield were improved by 29.23% and 33.31%,respectively. It was shown that exogenous silicon fertilizer can strengthen the ascorbic acid and glutathione circulatory system in flag leaf,enhance the plant's ability of anti-drought stress and increase the yield of grain. |
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