| 穆京妹，王琪琳，许 鑫，薛文波，吴建辉， 詹刚明，黄丽丽，康振生，韩德俊.小麦抗源南农790成株期抗条锈性遗传分析与分子作图[J].麦类作物学报,2015,35(3):306 |
| 小麦抗源南农790成株期抗条锈性遗传分析与分子作图 |
| Genetic Analysis and Molecular Mapping of Stripe Rust Resistant Gene in Wheat Line Nannong 790 |
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| DOI：10.7606/j.issn.1009-1041.2015.03.03 |
| 中文关键词: 小麦 条锈病 抗条锈基因 遗传分析 分子作图 |
| 英文关键词:Wheat Stripe Rust Resistance gene Genetic analysis Molecular mapping |
| 基金项目:国家高技术研究发展计划(863计划)项目(2012AA101503)；国家自然科学基金项目(31371924)；国家小麦产业技术体系研究项目(CARS 3 1 11)；高等学校学科创新引智计划项目(B07049) |
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| 中文摘要: |
| 为明确小麦抗锈种质南农790条锈病抗性特征和抗病遗传规律，以5个条锈菌生理小种CYR23、CYR29、CYR31、CYR32和CYR33对其进行苗期抗谱鉴定，并以CYR32和CYR33混合小种对南农790、Avocet S及南农790与Avocet S正交所获得的F1代、F2代和F2∶3群体进行混合小种成株期抗条锈性鉴定及遗传分析。抗病鉴定结果显示，南农790苗期对CYR32和CYR33表现为中度感病（IT:6~7），对其余小种表现为抗病（IT≤2），成株期对混合小种表现为近免疫（IT≤2，S≤5％），表明南农790具有成株期抗条锈性；各世代抗病遗传分析结果表明，其成株期抗病性由一个显性单基因控制，暂命名为YrNan；采用集群分离分析法（BSA），以SSR分子标记对F2代分离群体进行分子作图，发现了6个与YrNan连锁的 SSR标记( Xgwm11， Xbarc187， Xbarc181， Xgwm273， Xwmc419和 Xwmc216)，并将其定位于小麦的1BL染色体，两侧最近的标记分别为 Xbarc181(1.3 cM)和 Xgwm273(6.3 cM)。研究结果为南农790抗条锈病基因的分子标记辅助选择及在育种上的应用提供了依据。 |
| 英文摘要: |
| Nannong 790 with a high level adult resistance to stripe rust is a potential resistant source. The objectives of this study were to characterize the stripe rust resistant gene in Nannong 790 and identify tightly linked molecular markers for marker assisted selection (MAS). A segregation population was derived from a cross between Nannong 790 and a susceptible cultivar Avocet S. Five Chinese stripe rust races (CYR23,CYR29,CYR31,CYR32 and CYR33) were used to evaluate the resistant spectral at seedling stage and two races (CYR32 and CYR33) were used to test Nannong 790,Avocet S and the F1,F2 and F2∶3 populations. The results showed Nannong 790 displayed middle susceptible to CYR32 and CYR33 at seedling stage(IT:6 7),while displayed nearly immune（IT≤2，S≤5％）at adult stage. It indicated that Nannong 790 was adult plant resistant source. Genetic analysis with F1,F2 and F2∶3 populations showed that the resistance in Nannong 790 was controlled by a single dominant gene,designated as YrNan temporarily. A total of 159 F2∶3 lines and 420 SSR markers were employed to map the resistant gene by bulked segregation analysis (BSA). The result showed that YrNan was localized to chromosome 1B and linked to six publicly available wheat SSR markers ( Xgwm11, Xbarc187, Xbarc181, Xgwm273, Xwmc419 and Xwmc216),and the two closed flanked markers were Xbarc181 and Xgwm273 at genetic distances of 1.3 cM and 6.3 cM,respectively. It would facilitate the marker assisted selection (MAS) and the applying of YrNan in wheat breeding programs. |
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