| In order to know the changes of leaf cuticular wax composition and ultrastructure during the developmental growth of Aegilops tauschii, leaves were harvested as the test materials to extract the cuticular waxes. The wax composition and content were qualitatively and quantatively analyzed with gas chromatography-mass spectrometry (GC-MS) and gas chromatography-flame ionization detector (GC-FID), and the ultrastructure of leaf cuticular waxes was observed by scanning electron microscope. The results showed that 21 chemical compounds were identified by GC-MS analysis, and the main components of cuticualr waxes were primary alcohols (seedling stage 85%, heading stage 84% and filling stage 70%), with small amounts of alkanes, fatty acids, aldehydes and diketones. With the developmental growth of Aegilops tauschii, the total wax was constantly synthesized, the content of alkanes increased significantly, among which the increase of C29 alkanes was the most significant. however, the content of primary alcohols reduced significantly, especially the relative amount of C26 alcohol (seedling stage 80%, heading stage76%, filling stage 63%), the changes of other components were not obvious. Based on the results of scanning electron microscope, the wax crystal structures of adaxial and abaxial surface were in similar shape of platelets in these three stages. In addition, wax crystals on leaves at heading stage were sparse compared with waxes on leaves at seedling stage and became much sparser at filling stage, which could be caused by the increased amount of alkanes and the decreased amount of alcohols at these stages. |
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