| 赵翠荣，唐 清，王立峰，刘 莹，杨 青，彭 萍，陈科海，左珍喜，余华强，陈新民.秋水仙素及辅助处理对小麦×玉米单倍体加倍的影响[J].麦类作物学报,2014,34(11):1485 |
| 秋水仙素及辅助处理对小麦×玉米单倍体加倍的影响 |
| Effect of Different Colchicine Treatments and Supplementary Treatments on Doubled Haploid Production from Wheat × Maize |
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| DOI：10.7606/j.issn.1009-1041.2014.11.06 |
| 中文关键词: 小麦×玉米 单倍体 秋水仙素 辅助处理 染色体加倍 |
| 英文关键词:Wheat × maize Haploid Colchicine Supplementary treatments Chromosome doubling |
| 基金项目:国家小麦产业技术体系建设项目（CARS-03-01A）；主要粮食作物产业化湖北省协同创新中心资助项目；湖北省小麦工程技术研究中心建设项目；湖北省农业科技创新中心资助项目（2007-620-001-03） |
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| 摘要点击次数: 1945 |
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| 中文摘要: |
| 为了寻找方便、安全、高效的小麦单倍体苗加倍处理方法，选用秋水仙素3个浓度（0.1%、0.2%、0.3%）、3个处理时间（3 h、4 h、5 h）对单倍体苗进行浸根加倍处理；同时，比较了加光与不加光、全程通气与不通气、分蘖节打孔与不打孔等技术措施下单倍体苗的移栽成活率、加倍结实率。结果表明，室温下以0.2%的秋水仙素浸根处理3～4 h，期间持续通入空气并进行遮光处理的效果最佳，可获得90.0%的加倍率。避光和持续通气处理有利于提高成活率和加倍率，其在高浓度加倍处理和长时间加倍处理时显得尤其重要。分蘖节打孔伤害了植株，降低了加倍后植株的成活率，但能提高低浓度短时间处理时植株的加倍率，对低浓度长时间处理和高浓度处理则不适用。 |
| 英文摘要: |
| To explore a convenient, safe and efficient wheat haploid plantlets doubling method, three concentrations of colchicine(0.1%, 0.2%, 0.3%) and three processing durations(3 h, 4 h, 5 h), with supplementary treatments of light or darkness, pumping into air or not, drilling tiller node or not, and root-soaking treatments were used in this study to investigate their effects on doubling of the haploid plantlets from wheat × maize. The results showed that 90% of doubling frequency was obtained under the treatment of root-soaking for 3～4 h with 0.2% Colchicine and 30 mL·L-1 DMSO, in combination with pumping into air and no lights throughout the procedure. Darkness and continuous aeration in the whole process could improve both survival rate and doubling frequency, which was especially important when a high colchicine concentration with prolonged processing duration was applied. Tiller node drilling process somewhat hurted the treated plants and consequently reduced survival rate, while increased doubling frequency at low colchicine concentration with short processing duration; however, this treatment was not useful for time-prolonged treatments using either low or high concentrations of colchicine. |
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